The synthesis of a particle-forming cellular protein is enhanced by Mengo virus infection

Abstract

We have detected a cellular protein which not only escapes the shutoff of host translation induced by Mengo virus, but is synthesized in increasing amounts during Mengo virus infection. The protein has an apparent molecular weight of 20,000 and is contained in a remarkably stable cytoplasmic particle with a sedimentation coefficient of approximately 16 S in sucrose gradients. In the electron microscope this particle appears as a sphere of approximately 12 nm diameter. The synthesis of the protein is stimulated in mouse L cells and in HeLa cells infected with Mengo virus. Its synthesis cannot be induced, however, by stress (heat) or by infection with reovirus.