Difference in extracellular radical release after chemotactic factor and calcium ionophore activation of the oxygen radical-generating system in human neutrophils

Abstract

Results obtained with the luminol-dependent chemiluminescence technique show that with this technique, generation of radicals from an extra- as well as from an intracellular source is quantified. By means of a chemiluminescence technique, using human neutrophils stimulated with the chemoattractant formylmethionylleucylphenylalanine and the calcium ionophore ionomycin, two different mechanisms of radical production and release are demonstrated. The chemoattractant causes the cells to produce oxygen radicals which to a large extent are released from the cells. The calcium ionophore is also capable of stimulating radical formation but does not suffice for extracellular release. Furthermore, the removal of extracellular Ca2+ is of minor importance for the extracellular radical production, whereas it totally inhibits the generation of radicals with an intracellular localization. The mechanism(s) behind intracellular and extracellular production of oxygen radicals is discussed.