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. 2018 Nov;16(5):4075-4081.
doi: 10.3892/etm.2018.6723. Epub 2018 Sep 11.

Expression of tissue factor in human cervical carcinoma tissue

Xitong Zhao  1 , Chu Cheng  1 , Jinhai Gou  1 , Tao Yi  1 , Yanping Qian  1 , Xue Du  1 , Xia Zhao  1
Affiliations

Expression of tissue factor in human cervical carcinoma tissue

Xitong Zhao et al. Exp Ther Med. 2018 Nov.

Abstract

The present study aimed to investigate tissue factor (TF) expression in cervical cancer and explore its association with disease progression. A total of 258 cervical cancer tissues and their adjacent normal tissues were collected between September 2014 and September 2016. TF expression was detected in the tissue samples by immunohistochemistry and western blot analysis. Associations between the expression of TF and clinical stage, differentiation status and metastasis of cancer cells were examined. The mean immunohistochemistry score of TF expression in cervical cancer tissues was 2.86±1.76, which was significantly increased compared with the adjacent normal tissues (0.28±0.45). The expression of TF was also significantly associated with the clinical stage, lymph node metastasis and distant metastasis of cancer cells. Immunohistochemistry staining and western blot analysis demonstrated that TF expression in cervical cancer tissues significantly increased as the clinical stage increased. TF expression in tumor tissues from patients with lymph node metastasis was significantly increased compared with samples from patients without lymph node metastasis. TF expression was also significantly increased in patients with distant metastasis compared with those without. In conclusion, TF is highly expressed in cervical cancer tissues and high expression of TF may enhance the invasion and metastasis of cervical cancer cells.

Keywords: cervical cancer; metastasis; tissue factor.

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Figures

Figure 1.
Figure 1.
Detection of TF expression by immunohistochemistry. (A) Low TF expression in cervical cancer tissue. (B) High TF expression in cervical cancer tissue. (C) Negative TF expression in adjacent normal tissue. (D) Low TF expression in adjacent normal tissue. Magnification, ×200. (E) Statistical analysis of the immunohistochemistry results. TF, tissue factor.
Figure 2.
Figure 2.
TF expression is elevated in association with the clinical stage of cervical cancer. Expression of TF in stage (A) Ia1-Ib2, (B) IIa1-IIb and (C) IIIa cervical cancer tissue. Magnification, ×200. (D) TF protein expression in tumor tissues at different clinical stages of cervical cancer as determined by western blot analysis. Statistical analysis of the (E) immunohistochemistry and (F) western blotting. TF, tissue factor.
Figure 3.
Figure 3.
Association of TF expression with cervical cancer cell lymph node metastasis. (A and B) Detection of TF in cervical cancer tissues of two representative patients with LNM by immunohistochemistry. (C and D) Detection of TF in cervical cancer tissues of two representative patients LNUM by immunohistochemistry. Magnification, ×200. (E) Examination of TF expression in tumor tissue from patients with and without LNM as determined by western blot analysis. Statistical analysis of (F) immunohistochemistry and (G) western blotting. LNM, lymph node metastasis; LNUM, without lymph node metastasis; TF, tissue factor.
Figure 4.
Figure 4.
Association of TF expression with distal metastasis of cervical cancer cells. (A and B) TF expression in two representative cases of cervical cancer with DM as detected by immunohistochemistry. (C and D) TF expression in two representative cases of cervical cancer NDM as detected by immunohistochemistry. Magnification, ×200. (E) TF protein expression in tumor tissue of patients with and without DM as determined by western blotting. Statistical analysis of (F) immunohistochemistry and (G) western blotting. DM, distal metastasis; TF, tissue factor; NDM, without distal metastasis.
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