Receptors for IgM and IgM-antigen complexes on human T-lymphocytes reacting with specific anti-human-T-cell antiserum

Abstract

A receptor on T lymphocytes that can bind IgM-type antibodies, without, as well as after prior complex formation between the antibody and Esh (sheep red blood cells) has been detected. No prior in vitro incubation of freshly drawn and isolated peripheral blood lymphocytes is required for the expression of the IgM antibody binding receptor on T cells. Results obtained in experiments designed for the simultaneous detection of lymphocytes reacting with specific rhodamine labelled antihuman-T cell antiserum and forming rosettes with EA-IgM complexes of Esh and the IgM antiserum fraction showed the receptor for IgM antibody to be present exclusively on T cells. Pre-incubation of T cells with free IgM or its Fc fragments leads to inhibition of rosette formation with EA-IgM. Treatment of the IgM type pentameric antibody with dithioerythritol to cleave pentameric IgM reduces the percentage of EA-IgM rosette forming cells (RFC) significantly. The possible biological significance of this receptor is discussed.