Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Nov 6;23(11):2888.
doi: 10.3390/molecules23112888.

Immobilized Cells of Bacillus circulans ATCC 21783 on Palm Curtain for Fermentation in 5 L Fermentation Tanks

Jinpeng Wang  1   2 , Yao Hu  3   4 , Chao Qiu  5   6 , Haoran Fan  7   8 , Yan Yue  9   10 , Aiquan Jiao  11   12   13 , Xueming Xu  14   15 , Zhengyu Jin  16   17   18
Affiliations

Immobilized Cells of Bacillus circulans ATCC 21783 on Palm Curtain for Fermentation in 5 L Fermentation Tanks

Jinpeng Wang et al. Molecules. .

Abstract

Palm curtain was selected as carrier to immobilize Bacillus circulans ATCC 21783 to produce β-cyclodextrin (β-CD). The influence for immobilization to CGTase activity was analyzed to determine the operation stability. 83.5% cyclodextrin glycosyltransferases (CGTase) of the 1st cycle could be produced in the 7th cycle for immobilized cells, while only 28.90% CGTase was produced with free cells. When palm curtain immobilized cells were reused at the 2th cycle, enzyme activities were increased from 5003 to 5132 U/mL, which was mainly due to physical adsorption of cells on palm curtain with special concave surface structure. Furthermore, conditions for expanded culture of immobilized cells in a 5 L fermentation tank were optimized through specific rotation speed procedure (from 350 r/min to 450 r/min with step size of 50 r/min) and fixed ventilation capacity (4.5 L/min), relations between biomass, enzyme activity, pH, and oxygen dissolution was investigated, and the fermentation periods under the two conditions were both 4 h shorter. Compared with free cell, immobilized cell was more stable, effective, and had better application potential in industries.

Keywords: CGTase; cell immobilization; cyclodextrin; fermentation; palm curtain.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SEM images of different matrices (Page: 3A,B) initial palm curtain with magnifications of ×5000 and ×1000, respectively; (Page: 3C,D) Bacillus circulans ATCC 21783 immobilized on palm curtain with magnifications of ×10,000 and ×5000, respectively; (E) initial loofa sponge with magnification of ×500; (F) Bacillus circulans ATCC 21783 immobilized on loofa sponge with magnification of ×5000.
Figure 2
Figure 2
Cyclodextrin production by free and palm curtain immobilized Bacillus circulans ATCC 21783.
Figure 3
Figure 3
Effect of immobilization time (A), initial biomass (B), temperature (C), and pH (D) on the production of palm curtain immobilized Bacillus circulans ATCC 21783 enzyme activity.
Figure 4
Figure 4
Reusability of different matrices immobilized Bacillus circulans ATCC 21783 (* means the significant difference between palm curtain and loofa sponge, p < 0.05).
Figure 5
Figure 5
Biomass, enzyme activity, pH, and oxygen dissolution during cultivation of palm curtain immobilized Bacillus circulans ATCC 21783 in a 5 L tank (A) relation of biomass and enzyme activity; (B) relation of biomass and pH; (C) relation of enzyme activity and pH; (D) relation of biomass and oxygen dissolution.
Figure 6
Figure 6
Biomass, oxygen dissolution, enzyme activity and pH variation of palm curtain immobilized Bacillus circulans ATCC 21783 by increasing the rotation speed (A) relation of biomass and oxygen dissolution; (B) relation of biomass and enzyme activity; (C) relation of biomass and pH; (D) relation of pH and enzyme activity.
Figure 7
Figure 7
Effects of ventilation on enzyme activity during cultivation of palm curtain immobilized Bacillus circulans ATCC 21783 in a 5 L tank.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Atanasova N., Petrova P., Ivanova V., Yankov D., Vassileva A., Tonkova A. Isolation of novel alkaliphilic bacillus strains for cyclodextrin glucanotransferase production. Appl. Biochem. Biotechnol. 2008;149:155–167. doi: 10.1007/s12010-007-8128-5. - DOI - PubMed
    1. Kitahata S., Tsuyama N., Okada S. Purification and some properties of cyclodextrin glycosyltransferase from a strain of Bacillus species. Agric. Biol. Chem. 1974;38:387–393. doi: 10.1080/00021369.1974.10861152. - DOI
    1. Van der Veen B.A., Uitdehaag J.C.M., Dijkstra B.W., Dijkhuizen L. The role of arginine 47 in the cyclization and coupling reactions of cyclodextrin glycosyltransferase from Bacillus circulans strain 251. Eur. J. Biochem. 2000;267:3432–3441. doi: 10.1046/j.1432-1327.2000.01353.x. - DOI - PubMed
    1. Vassileva A., Atanasova N., Ivanova V., Dhulster P., Tonkova A. Characterisation of cyclodextrin glucanotransferase from Bacillus circulans ATCC 21783 in terms of cyclodextrin production. Ann. Microbiol. 2007;57:609–615. doi: 10.1007/BF03175362. - DOI
    1. Qi Q.S., Zimmermann W. Cyclodextrin glucanotransferase: From gene to applications. Appl. Microbiol. Biotechnol. 2005;66:475–485. doi: 10.1007/s00253-004-1781-5. - DOI - PubMed

MeSH terms

Substances

LinkOut - more resources