Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Sep;11(9):1250-1254.
doi: 10.14202/vetworld.2018.1250-1254. Epub 2018 Sep 12.

n-Propanol extract of boiled and fermented koro benguk (Mucuna pruriens seed) shows a neuroprotective effect in paraquat dichloride-induced Parkinson's disease rat model

Yosua Kristian Adi  1 , Rini Widayanti  2 , Tri Wahyu Pangestiningsih  3
Affiliations

n-Propanol extract of boiled and fermented koro benguk (Mucuna pruriens seed) shows a neuroprotective effect in paraquat dichloride-induced Parkinson's disease rat model

Yosua Kristian Adi et al. Vet World. 2018 Sep.

Abstract

Aim: n-Propanol extracts from fresh, boiled, and fermented seeds were studied to evaluate their neuroprotective effects in a Parkinson's disease (PD) rat model, based on the total number of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc).

Materials and methods: Rats were induced with paraquat dichloride at a dosage of 7 mg/kg BW intraperitoneally twice a week and at the same time supplemented with extract at a dosage of 70 mg/kg BW orally every day for 3 weeks. On the 24th day, all rats were perfused and fixed with 4% paraformaldehyde. The left part of the SNpc was processed for immunohistochemical staining with tyrosine hydroxylase (TH)-antibody. The total number of DA neurons in SNpc was evaluated with a stereological method.

Results: TH-immunoreactive cells found in the SNpc were identified as DA neurons. The average total number of DA neurons in the SNpc increased significantly in the PD rat model that was given an n-propanol extract of boiled and fermented seeds compared with a control PD rat model. Surprisingly, there was no significant difference in the average total number of DA neurons in SNpc between the PD rat model that was given n-propanol extract of fresh seeds and the control PD rat model.

Conclusion: n-Propanol extract of boiled and fermented seeds could produce a higher neuroprotective effect against DA neuron than fresh seeds in a PD rat model.

Keywords: Mucuna pruriens; Parkinson’s disease; animal model; neuroprotective; paraquat dichloride.

PubMed Disclaimer

Figures

Figure-1
Figure-1
Diagram showing the average total number of TH-IR cells in the SNpc of the five groups. The symbol * indicates a significant difference compared with Group I; # indicates a significant difference compared with Group II; $ indicates a significant difference compared with Group IIIA; € indicates a significant difference compared with Group IIIB; Δ indicates a significant difference compared with Group IIIC (p<0.05).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. WHO. The World Health Report 1998-Life in the 21st Century: A Vision for All. Geneva: World Health Organization; 1998.
    1. WHO. Neurological Disorders: Public Health Challenges. Geneva: World Health Organization; 2006.
    1. Mackenzie I.R.A. Neuropathology of atypical parkinsonian disorders. In: Litvan I, editor. Atypical Parkinsonian Disorders. Current Clinical Neurology. Philadelphia, PA: Humana Press; 2005.
    1. Uversky V.N. Neurotoxicant-induced animal models of Parkinson's disease: Understanding the role of rotenone, maneb, and paraquat in neurodegeneration. Cell Tissue Res. 2004;318:225–241. - PubMed
    1. Logroscino G. The Role of early life environmental risk factors in Parkinson disease: What is the evidence? Environ. Health Perspect. 2005;113(9):1234–1238. - PMC - PubMed

LinkOut - more resources