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Review
. 2018 Nov 8;10(4):221.
doi: 10.3390/pharmaceutics10040221.

A Review on Liquid Chromatography-Tandem Mass Spectrometry Methods for Rapid Quantification of Oncology Drugs

Andrea Li-Ann Wong  1   2 , Xiaoqiang Xiang  3 , Pei Shi Ong  4 , Ee Qin Ying Mitchell  5 , Nicholas Syn  6   7 , Ian Wee  8   9 , Alan Prem Kumar  10   11 , Wei Peng Yong  12   13 , Gautam Sethi  14 , Boon Cher Goh  15   16   17 , Paul Chi-Lui Ho  18 , Lingzhi Wang  19   20
Affiliations
Review

A Review on Liquid Chromatography-Tandem Mass Spectrometry Methods for Rapid Quantification of Oncology Drugs

Andrea Li-Ann Wong et al. Pharmaceutics. .

Abstract

In the last decade, the tremendous improvement in the sensitivity and also affordability of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has revolutionized its application in pharmaceutical analysis, resulting in widespread employment of LC-MS/MS in determining pharmaceutical compounds, including anticancer drugs in pharmaceutical research and also industries. Currently, LC-MS/MS has been widely used to quantify small molecule oncology drugs in various biological matrices to support preclinical and clinical pharmacokinetic studies in R&D of oncology drugs. This mini-review article will describe the state-of-the-art LC-MS/MS and its application in rapid quantification of small molecule anticancer drugs. In addition, efforts have also been made in this review to address several key aspects in the development of rapid LC-MS/MS methods, including sample preparation, chromatographic separation, and matrix effect evaluation.

Keywords: LC-MS/MS; oncology drugs; quantification; rapid.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Pipeline for conducting bioanalysis using liquid chromatography-tandem mass spectrometry.
Figure 2
Figure 2
Distribution of sample preparation assays. PPT: protein precipitation; LLE: liquid-liquid extraction; SPE: solid phase extraction.
Figure 3
Figure 3
Run time (min) for determination of 1 analyte.
Figure 4
Figure 4
Run time (min) for determination of ≥2 analytes.
Figure 5
Figure 5
The schematic of the post-column infusion system for evaluation of matrix effects.
See this image and copyright information in PMC

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