Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Jul 11;16(13):5863-78.
doi: 10.1093/nar/16.13.5863.

Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis

M Tommasino  1 , S Ricci, C L Galeotti
Affiliations
Free PMC article

Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis

M Tommasino et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have determined the entire sequence of the plasmid K2 from Kluyveromyces lactis which is involved in the maintenance of both killer plasmids in the cell. K2 shares many of the characteristics of the smaller killer plasmid K1: high A+T content (74.7%) and very compact genomic organization. K2 contains ten open reading frames. Some of them overlap on different strands and some on the same strand. Northern blotting of K2 transcripts shows that at least eight ORFs are transcribed. Analysis of the predicted aminoacid sequence of ORF2 from K2 reveals homology with the aminoacid sequence of ORF 1 from K1 and with several viral DNA polymerases. The sequence of K2 from Saccaromyces cerevisiae F102-2 was also determined. Only one nucleotide difference was found between the K2 sequence from the two yeasts. This mutation does not change the genome organization of the plasmid and has only minimal effect on the structure of the encoded proteins.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1986 Sep 11;14(17):6871-84 - PubMed
    1. Nucleic Acids Res. 1985 Nov 25;13(22):8143-63 - PubMed
    1. Virology. 1986 Mar;149(2):208-16 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 May;82(9):2829-33 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5225-9 - PubMed

Associated data