Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Jul 25;16(14A):6309-26.
doi: 10.1093/nar/16.14.6309.

Effects of macromolecular crowding on the association of E. coli ribosomal particles

S B Zimmerman  1 , S O Trach
Affiliations
Free PMC article

Effects of macromolecular crowding on the association of E. coli ribosomal particles

S B Zimmerman et al. Nucleic Acids Res. .
Free PMC article

Erratum in

  • Nucleic Acids Res 1988 Oct 25;16(20):9892

Abstract

The equilibrium for the binding reaction between the 30 S and 50 S ribosomal subunits of E. coli is shifted towards formation of 70 S ribosomes in the presence of a variety of polymers. The polymers also increase a further interaction between 70 S particles to form the 100 S dimer. The requirement for relatively high concentrations of non-specific polymers indicates that the shifts in equilibria arise from excluded volume effects. Analysis using scaled particle theory is consistent with this mechanism. The effects of high concentrations of polymers on the interactions between ribosomal species may make important changes in the function of ribosomes under the crowded conditions which occur in vivo.

PubMed Disclaimer

References

    1. J Mol Biol. 1977 May 25;112(3):437-52 - PubMed
    1. J Mol Biol. 1972 Nov 14;71(2):263-79 - PubMed
    1. J Mol Biol. 1973 Nov 5;80(3):519-29 - PubMed
    1. Eur J Biochem. 1970 Dec;17(3):433-40 - PubMed
    1. Anal Biochem. 1977 Oct;82(2):580-2 - PubMed