Flavobacterium edaphi sp. nov., isolated from soil from Jeju Island, Korea

Abstract

An aerobic, Gram-stain-negative, bright yellow-pigmented, oxidase and catalase-positive, non-motile, non-spore forming, rod-shaped strain designated DMN11^T was isolated from the soil of crossroads of Jeju Island in South Korea. Colonies were circular, bright yellow-pigmented and smooth with regular edges and measured approximately 1-2 mm in diameter. Flexirubin-type pigments were absent. Phylogenetic tree analysis based on the 16SrRNA gene sequence revealed that the strain DMN11^T formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes, and it was the most closely related to Flavobacterium suzhouense XIN-1^T and Flavobacterium hauense BX12^T (98.6% and 98.2% similarity, respectively). The major isoprenoid quinone was MK-6. The major fatty acids were summed feature 3 (comprising C_16:1ω7c and/or C_16:1ω6c), iso-C_15:0 and iso-C_15:0 3OH. The polar lipid profile of the strain DMN11^T showed the presence of phosphatidylethanolamine (PE) as major lipid. The DNA G+C content was 35.3 mol%, as determined by the thermal denaturation method. The mean levels of DNA-DNA relatedness of the strain DMN11^T with F. suzhouense XIN-1^T and F. hauense BX12^T were 20.5% and 29.2%, respectively. Thus, the data accumulated in this study support the suggestion that the strain DMN11^T is considered to represent a novel species of the genus Flavobacterieum, for which the name Flavobacterium edaphi sp. nov. is proposed. The type strain is DMN11^T (= KCTC 62114^T = JCM 32372^T).

Keywords: Chemotaxonomic approach; Crossroad soil; DNA relatedness; Flavobacteriaceae; Strictly aerobic.