doi: 10.1016/0022-1759(78)90002-9.
Studies on the platelet radioactive anti-immunoglobulin test
- PMID: 304464
- DOI: 10.1016/0022-1759(78)90002-9
Item in Clipboard
Studies on the platelet radioactive anti-immunoglobulin test
J Immunol Methods.
1978.
Abstract
A platelet radioactive antiimmunoglobulin test (PRAT) has recently been introduced. In the present report, a detailed analysis the influence of varying test conditions (i.e. efficiency of iodination of anti-IgG, cell number, degree of sensitization of platelets, times and temperatures of incubation, frequency of washings) on the results of this technique is presented. A standard procedure based on these findings is outlined. It is shown that the PRAT is two to four times more sensitive than platelet complement fixation for the detection of HLA antibodies.
Similar articles
-
Immunofluorescence microphotometry for the detection of platelet antibodies. II. Sensitivity of the method compared with that of conventional serological methods.Scand J Immunol. 1973;2(5):531-9. doi: 10.1111/j.1365-3083.1973.tb02059.x. Scand J Immunol. 1973. PMID: 4590756 No abstract available.
-
HLA--C antigens on platelets.Tissue Antigens. 1980 Jul;16(1):91-4. Tissue Antigens. 1980. PMID: 6970427
-
125I anti-immunoglobulin binding assay for the detection and characterization of anti-platelet antibodies.Hum Immunol. 1980 Dec;1(4):369-81. doi: 10.1016/0198-8859(80)90113-5. Hum Immunol. 1980. PMID: 7021489
-
[HLA and transfusion: new approaches with Luminex™ technology].Transfus Clin Biol. 2011 Apr;18(2):218-23. doi: 10.1016/j.tracli.2011.01.004. Epub 2011 Mar 11. Transfus Clin Biol. 2011. PMID: 21397543 Review. French.
-
The measurement of platelet-bound immunoglobulins: an overview of the methods and the biological relevance of platelet-associated IgG.Prog Hematol. 1983;13:163-99. Prog Hematol. 1983. PMID: 6366912 Review. No abstract available.
Cited by
-
Stimulation of lymphocytes by platelet-antibody-complexes and their role in the pathogenesis of idiopathic thrombocytopenia.Blut. 1979 Jul;39(1):53-60. doi: 10.1007/BF01008075. Blut. 1979. PMID: 572717
-
The role of alloimmunization in platelet survival studies.Blut. 1980 Aug;41(2):93-9. doi: 10.1007/BF01039653. Blut. 1980. PMID: 7437528
-
Platelet surface antigens: analysis by monoclonal antibodies. I. Antibodies. I. Immunological and biochemical studies.Blut. 1984 Mar;48(3):161-70. doi: 10.1007/BF00320339. Blut. 1984. PMID: 6199057
-
Immunological studies in a case of Evans' syndrome.Blut. 1981 Jan;42(1):27-32. doi: 10.1007/BF01080261. Blut. 1981. PMID: 7194128
-
Post-transfusion thrombocytopenic purpura: immunological and clinical studies in two cases and review of the literature.Blut. 1980 Apr;40(4):249-57. doi: 10.1007/BF01080184. Blut. 1980. PMID: 7189423
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
