Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

Shigeki Uchinuma¹, Yasushi Shimada^{1

2}, Khairul Matin^{3

4}, Keiichi Hosaka¹, Masahiro Yoshiyama², Yasunori Sumi⁵, Junji Tagami¹

Affiliations

¹ Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-4-45, Yushima, Bunkyou-ku, Tokyo, 113-8549, Japan.
² Department of Operative Dentistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
³ Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-4-45, Yushima, Bunkyou-ku, Tokyo, 113-8549, Japan. matin.ope@tmd.ac.jp.
⁴ Endowed Department of International Oral Health Science, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Kanagawa, 230-0063, Japan. matin.ope@tmd.ac.jp.
⁵ National Center for Geriatrics and Gerontology, 7-430, Morioka-cho, Obu City, Aichi, 474-8511, Japan.

PMID: 30448940
DOI: 10.1007/s10103-018-2685-4

Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

Shigeki Uchinuma et al. Lasers Med Sci. 2019 Jul.

. 2019 Jul;34(5):981-989.

doi: 10.1007/s10103-018-2685-4. Epub 2018 Nov 17.

Authors

Shigeki Uchinuma¹, Yasushi Shimada^{1

2}, Khairul Matin^{3

4}, Keiichi Hosaka¹, Masahiro Yoshiyama², Yasunori Sumi⁵, Junji Tagami¹

Affiliations

¹ Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-4-45, Yushima, Bunkyou-ku, Tokyo, 113-8549, Japan.
² Department of Operative Dentistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
³ Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-4-45, Yushima, Bunkyou-ku, Tokyo, 113-8549, Japan. matin.ope@tmd.ac.jp.
⁴ Endowed Department of International Oral Health Science, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Kanagawa, 230-0063, Japan. matin.ope@tmd.ac.jp.
⁵ National Center for Geriatrics and Gerontology, 7-430, Morioka-cho, Obu City, Aichi, 474-8511, Japan.

PMID: 30448940
DOI: 10.1007/s10103-018-2685-4

Abstract

The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD₄₉₀) were also taken after irradiation. In addition, the bactericidal effects of irradiation against S. mutans under 0.5 mm-thick dentin were compared using culture tests and OD₄₉₀ measurements. Direct UV-LED irradiation with both UVB and UVC showed strong bactericidal effects. UVB showed superior bactericidal effect through 0.5-mm-thick dentin than did UVC, especially after demineralization. These results suggest that UVB irradiation could be utilized for the prevention and management of dental caries.

Keywords: Demineralized dentin; Streptococcus mutans; Streptococcus sobrinus; UV-LED; UVB; UVC.

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Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

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Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

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