Data on SD-OCT image acquisition, ultrastructural features, and horizontal tissue shrinkage in the porcine retina

Abstract

The data presented in this article are related to the research paper entitled "Correlation of Spectral Domain Optical Coherence Tomography with Histology and Electron Microscopy in the Porcine Retina" (Xie et al., 2018) [2]. This research data highlights our technique for retinal fundus image acquisition during spectral domain optical coherence tomography (SD-OCT) in a large animal model. Low and high magnification electron micrographs are included to demonstrate the ultrastructural features of the porcine retina. Data on horizontal tissue shrinkage during processing of the porcine retina are presented.

Fig. 1

Infrared and blue reflectance imaging of the porcine retina with alignment for OCT image acquisition. Porcine fundus images were acquired with infrared reflectance imaging (A) and blue reflectance imaging (B). Note the speckled pattern of choroidal pigment in the infrared image (A) and the high definition details of the retinal vasculature and the retinal nerve fiber layer (asterisk) in the blue reflectance image (B). Eye positioning during SD-OCT image acquisition can induce errors. The animal should be positioned with the superior retina located superiorly and the optic nerve head located inferiorly in the infrared image for OCT imaging as shown in A.

Fig. 2

TEM of endothelial cells and fibrous astrocyte in the inner plexiform layer. High magnification TEM (A) of upper left boxed area in Xie et al., 2018 (Figure 4A) showing endothelial cells with junctional densities (arrows) and basement membrane (arrowheads). High magnification TEM (B) of upper right boxed area in Xie et al., 2018 (Figure 4A) shows a fibrous astrocyte with patchy basement membrane (arrow), subplasmalemmal aggregates of microfilaments (arrowhead), and fine collagenous fibrils (asterisk) arranged perpendicular to the basement and plasma membranes. These cell types, in addition to amacrine and Müller cells, corresponded to a discontinuous relative hypo-reflective band in the inner plexiform layer. Scale bar: 1 micron.

Fig. 3

TEM of amacrine and müller cells in the inner plexiform layer. High magnification TEM of an amacrine cell (A) and a Müller cell (B) located in the middle aspect of the inner plexiform layer. Note the lucent cytoplasm (asterisks), dispersed nucleoplasm, and small nucleolus (arrow) in the amacrine cell (A) and the extensive network of relatively electron dense cytoplasmic processes (arrowhead) of the Müller cell wrapping around other cytoplasmic processes in the inner plexiform layer. These cell types, in addition to endothelial cells and fibrous astrocytes, appeared to correspond to a discontinuous relative hypo-reflective band in the inner plexiform layer. Scale bars: A, 2 microns; B, 5 microns.

Fig. 4

Low magnification TEM of the porcine retina. Note the large horizontal cell (HC) in the outer aspect of the inner nuclear layer (INL), the Müller cell body (MC) in the inner aspect of the INL, and the deep retinal capillaries (arrowheads). Cone nuclei (asterisk) were concentrated in the outer aspect of the outer nuclear layer (ONL) and corresponded to a relative hypo-reflective band in the ONL. Other abbreviations: IPL, inner plexiform layer; OPL, outer plexiform layer; IS, inner segments; OS, outer segments; CH, choroid. Scale bar: 10 microns.

Fig. 5

TEM of bipolar cell and horizontal cell cytoplasm. High magnification TEM of boxed area in Xie et al. [2] (Fig. 4C) shows numerous mitochondria (M) in bipolar (BC) and horizontal (HC) cells in the inner nuclear layer. The mitochondria in these cells appeared to correspond to a hyper-reflective band in the middle of the inner nuclear layer on SD-OCT. Scale bar: 1 micron.

Fig. 6

TEM of outer retina and retinal pigment epithelium. The RPE cells exhibited long apical cytoplasmic cell processes (arrowheads) which were intimately associated with the photoreceptor outer segments in the interdigitation zone. Some of the RPE apical cell processes appeared to extend to near the photoreceptor inner-outer segment junction. Abbreviations: cone nuclei, CN; external limiting membrane, ELM; cone ellipsoid, CE; retinal pigment epithelium, RPE. Scale bar: 10 microns.

Fig. 7

SD-OCT-histology correlation and horizontal tissue shrinkage. Infrared reflectance imaging of porcine retina (A) demonstrates the scanned area (white line) in the superior retina, including a branch retinal vein (asterisk) and a branch retinal arteriole (arrow). Note the superior margin of the optic nerve head (arrowheads). The SD-OCT image (B) shows the same larger (asterisk) and smaller (arrow) retinal vessels displacing the internal limiting membrane (ILM) toward the vitreous. A toluidine blue-stained epoxy resin embedded section (C) obtained from the corresponding retina reveals that the larger vessel (asterisk) is a large branch retinal venule and the smaller vessel (arrow) is a small branch retinal arteriole. Note the corresponding inward displacement of the ILM toward the vitreous by the retinal vessels in the SD-OCT and histologic images (B, C). There is apparent lateral or horizontal (but not vertical) tissue shrinkage (B, C) after histologic processing. Incongruity in the measurements of the horizontal length of the retina in the OCT image vs. the photomicrograph might be due, in part, to image stretching or distortion associated with SD-OCT algorithmic image processing. Scale bars: A, 500 microns; B and C, 200 microns.