Cloning and nucleotide sequence of the oriT region of the IncI1 plasmid R64
- PMID: 3045094
- PMCID: PMC211456
- DOI: 10.1128/jb.170.9.4385-4387.1988
Cloning and nucleotide sequence of the oriT region of the IncI1 plasmid R64
Abstract
The nucleotide sequence at the oriT region of the IncI1 plasmid R64 was determined. A recombinant plasmid carrying a 141-base-pair R64 sequence was mobilized with a normal frequency, while a plasmid carrying only 44 base pairs of this R64 sequence was mobilized with a frequency 1/10 that of the original plasmid. The oriT region of the R64 plasmid contains two inverted-repeat sequences.
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