Genetic Mechanisms behind the Spread of Reduced Susceptibility to Azithromycin in Shigella Strains Isolated from Men Who Have Sex with Men in Québec, Canada

doi:10.1128/AAC.01679-18

. 2019 Jan 29;63(2):e01679-18.

doi: 10.1128/AAC.01679-18. Print 2019 Feb.

Genetic Mechanisms behind the Spread of Reduced Susceptibility to Azithromycin in Shigella Strains Isolated from Men Who Have Sex with Men in Québec, Canada

Khadidja Yousfi¹, Christiane Gaudreau^{2

3}, Pierre A Pilon^{1

4}, Brigitte Lefebvre¹, Matthew Walker⁵, Éric Fournier¹, Florence Doualla Bell¹, Christine Martineau^{1

2}, Jean Longtin¹, Sadjia Bekal^{6

2}

Affiliations

¹ Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Canada.
² Université de Montréal, Québec, Canada.
³ Centre Hospitalier de l'Université, Montréal, Québec, Canada.
⁴ Direction Régionale de Santé Publique de Montréal, Québec, Canada.
⁵ National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
⁶ Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Canada sadjia.bekal@inspq.qc.ca.

PMID: 30455248
PMCID: PMC6355565
DOI: 10.1128/AAC.01679-18

Genetic Mechanisms behind the Spread of Reduced Susceptibility to Azithromycin in Shigella Strains Isolated from Men Who Have Sex with Men in Québec, Canada

Khadidja Yousfi et al. Antimicrob Agents Chemother. 2019.

. 2019 Jan 29;63(2):e01679-18.

doi: 10.1128/AAC.01679-18. Print 2019 Feb.

Authors

Affiliations

¹ Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Canada.
² Université de Montréal, Québec, Canada.
³ Centre Hospitalier de l'Université, Montréal, Québec, Canada.
⁴ Direction Régionale de Santé Publique de Montréal, Québec, Canada.
⁵ National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
⁶ Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Canada sadjia.bekal@inspq.qc.ca.

PMID: 30455248
PMCID: PMC6355565
DOI: 10.1128/AAC.01679-18

Abstract

We analyzed 254 Shigella species isolates collected in Québec, Canada, during 2013 and 2014. Overall, 23.6% of isolates showed reduced susceptibility to azithromycin (RSA) encoded by mphA (11.6%), ermB (1.7%), or both genes (86.7%). Shigella strains with RSA were mostly isolated from men who have sex with men (68.8% or higher) from the Montreal region. A complete sequence analysis of six selected plasmids from Shigella sonnei and different serotypes of Shigella flexneri emphasized the role of IS26 in the dissemination of RSA.

Keywords: IS26 insertion sequence; Shigella spp.; plasmid diversity; reduced susceptibility to azithromycin.

PubMed Disclaimer

Figures

**FIG 1**
Antimicrobial resistance regions on plasmids of *Shigella* with determinants of reduced susceptibility to azithromycin. (a) Genes are represented by arrows and colored according to gene function. Shaded regions represent shared structures harboring *mphA* and *ermB*. Dashed line represents the nonsequenced parts. (b) Comparison of the plasmids was performed using Gview server. The sizes of the plasmids are: 82,860 bp (pSf1), 79,827 bp (pSf2), 72,298 bp (pSf3), 67,764 bp (pSf4), 89,529 bp (pSf5), and 94,328 bp (pSs1). Gene organization diagrams were drawn using Inkscape v0.91.

See this image and copyright information in PMC

Cited by

A new plasmid carrying mphA causes prevalence of azithromycin resistance in enterotoxigenic Escherichia coli serogroup O6.
Xiang Y, Wu F, Chai Y, Xu X, Yang L, Tian S, Zhang H, Li Y, Yang C, Liu H, Qiu S, Song H, Sun Y. Xiang Y, et al. BMC Microbiol. 2020 Aug 11;20(1):247. doi: 10.1186/s12866-020-01927-z. BMC Microbiol. 2020. PMID: 32782021 Free PMC article.
Multiple Mechanisms Confer Resistance to Azithromycin in Shigella in Bangladesh: a Comprehensive Whole Genome-Based Approach.
Nusrin S, Asad A, Hayat S, Islam B, Begum R, Nabila FH, Islam Z. Nusrin S, et al. Microbiol Spectr. 2022 Aug 31;10(4):e0074122. doi: 10.1128/spectrum.00741-22. Epub 2022 Jul 25. Microbiol Spectr. 2022. PMID: 35876510 Free PMC article.
Acute Bacterial Gastroenteritis.
Fleckenstein JM, Matthew Kuhlmann F, Sheikh A. Fleckenstein JM, et al. Gastroenterol Clin North Am. 2021 Jun;50(2):283-304. doi: 10.1016/j.gtc.2021.02.002. Epub 2021 Apr 23. Gastroenterol Clin North Am. 2021. PMID: 34024442 Free PMC article. Review.
Mechanism of action, resistance, synergism, and clinical implications of azithromycin.
Heidary M, Ebrahimi Samangani A, Kargari A, Kiani Nejad A, Yashmi I, Motahar M, Taki E, Khoshnood S. Heidary M, et al. J Clin Lab Anal. 2022 Jun;36(6):e24427. doi: 10.1002/jcla.24427. Epub 2022 Apr 21. J Clin Lab Anal. 2022. PMID: 35447019 Free PMC article. Review.
The increasing antimicrobial resistance of Shigella species among Iranian pediatrics: a systematic review and meta-analysis.
Baharvand A, Molaeipour L, Alesaeidi S, Shaddel R, Mashatan N, Amiriani T, Kiaei Sudkolaei M, Abbasian S, Talib Al-Naqeeb BZ, Kouhsari E. Baharvand A, et al. Pathog Glob Health. 2023 Oct;117(7):611-622. doi: 10.1080/20477724.2023.2179451. Epub 2023 Feb 16. Pathog Glob Health. 2023. PMID: 36794800 Free PMC article.

See all "Cited by" articles

References

1. Committee on Infectious Diseases, American academy of Pediatrics. 2018. In Kimberlin DW, Brady MT, Jackson MA, Long SS. (ed) Red book: 2018 report of the Committee on Infectious Diseases, 31st ed American Academy of Pediatrics, Itasca, IL.
1. Baker KS, Dallman TJ, Ashton PM, Day M, Hughes G, Crook PD, Gilbart VL, Zittermann S, Allen VG, Howden BP, Tomita T, Valcanis M, Harris SR, Connor TR, Sintchenko V, Howard P, Brown JD, Petty NK, Gouali M, Thanh DP, Keddy KH, Smith AM, Talukder KA, Faruque SM, Parkhill J, Baker S, Weill FX, Jenkins C, Thomson NR. 2015. Intercontinental dissemination of azithromycin-resistant shigellosis through sexual transmission: a cross-sectional study. Lancet Infect Dis 15:913–921. doi:10.1016/S1473-3099(15)00002-X. - DOI - PubMed
1. Gaudreau C, Barkati S, Leduc JM, Pilon PA, Favreau J, Bekal S. 2014. Shigella spp. with reduced azithromycin susceptibility, Québec, Canada, 2012–2013. Emerg Infect Dis 20:854–856. doi:10.3201/eid2005.130966. - DOI - PMC - PubMed
1. Bowen A, Grass J, Bicknese A, Campbell D, Hurd J, Kirkcaldy RD. 2016. Elevated risk for antimicrobial drug resistant Shigella infection among men who have sex with men, United States, 2011–2015. Emerg Infect Dis 22:1613–1616. doi:10.3201/eid2209.160624. - DOI - PMC - PubMed
1. Gaudreau C, Bruneau A, Ismaïl J. 2005. Outbreak of Shigella flexneri and Shigella sonnei enterocolitis in men who have sex with men, Québec, 1999 to 2001. Can Commun Dis Rep 31:85–90. (In French.) - PubMed

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions

LinkOut - more resources

Full Text Sources
Miscellaneous
- NCI CPTAC Assay Portal

[1] Committee on Infectious Diseases, American academy of Pediatrics. 2018. In Kimberlin DW, Brady MT, Jackson MA, Long SS. (ed) Red book: 2018 report of the Committee on Infectious Diseases, 31st ed American Academy of Pediatrics, Itasca, IL.

[2] Committee on Infectious Diseases, American academy of Pediatrics. 2018. In Kimberlin DW, Brady MT, Jackson MA, Long SS. (ed) Red book: 2018 report of the Committee on Infectious Diseases, 31st ed American Academy of Pediatrics, Itasca, IL.

[3] Baker KS, Dallman TJ, Ashton PM, Day M, Hughes G, Crook PD, Gilbart VL, Zittermann S, Allen VG, Howden BP, Tomita T, Valcanis M, Harris SR, Connor TR, Sintchenko V, Howard P, Brown JD, Petty NK, Gouali M, Thanh DP, Keddy KH, Smith AM, Talukder KA, Faruque SM, Parkhill J, Baker S, Weill FX, Jenkins C, Thomson NR. 2015. Intercontinental dissemination of azithromycin-resistant shigellosis through sexual transmission: a cross-sectional study. Lancet Infect Dis 15:913–921. doi:10.1016/S1473-3099(15)00002-X. - DOI - PubMed

[4] Baker KS, Dallman TJ, Ashton PM, Day M, Hughes G, Crook PD, Gilbart VL, Zittermann S, Allen VG, Howden BP, Tomita T, Valcanis M, Harris SR, Connor TR, Sintchenko V, Howard P, Brown JD, Petty NK, Gouali M, Thanh DP, Keddy KH, Smith AM, Talukder KA, Faruque SM, Parkhill J, Baker S, Weill FX, Jenkins C, Thomson NR. 2015. Intercontinental dissemination of azithromycin-resistant shigellosis through sexual transmission: a cross-sectional study. Lancet Infect Dis 15:913–921. doi:10.1016/S1473-3099(15)00002-X. - DOI - PubMed

[5] Gaudreau C, Barkati S, Leduc JM, Pilon PA, Favreau J, Bekal S. 2014. Shigella spp. with reduced azithromycin susceptibility, Québec, Canada, 2012–2013. Emerg Infect Dis 20:854–856. doi:10.3201/eid2005.130966. - DOI - PMC - PubMed

[6] Gaudreau C, Barkati S, Leduc JM, Pilon PA, Favreau J, Bekal S. 2014. Shigella spp. with reduced azithromycin susceptibility, Québec, Canada, 2012–2013. Emerg Infect Dis 20:854–856. doi:10.3201/eid2005.130966. - DOI - PMC - PubMed

[7] Bowen A, Grass J, Bicknese A, Campbell D, Hurd J, Kirkcaldy RD. 2016. Elevated risk for antimicrobial drug resistant Shigella infection among men who have sex with men, United States, 2011–2015. Emerg Infect Dis 22:1613–1616. doi:10.3201/eid2209.160624. - DOI - PMC - PubMed

[8] Bowen A, Grass J, Bicknese A, Campbell D, Hurd J, Kirkcaldy RD. 2016. Elevated risk for antimicrobial drug resistant Shigella infection among men who have sex with men, United States, 2011–2015. Emerg Infect Dis 22:1613–1616. doi:10.3201/eid2209.160624. - DOI - PMC - PubMed

[9] Gaudreau C, Bruneau A, Ismaïl J. 2005. Outbreak of Shigella flexneri and Shigella sonnei enterocolitis in men who have sex with men, Québec, 1999 to 2001. Can Commun Dis Rep 31:85–90. (In French.) - PubMed

[10] Gaudreau C, Bruneau A, Ismaïl J. 2005. Outbreak of Shigella flexneri and Shigella sonnei enterocolitis in men who have sex with men, Québec, 1999 to 2001. Can Commun Dis Rep 31:85–90. (In French.) - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Genetic Mechanisms behind the Spread of Reduced Susceptibility to Azithromycin in Shigella Strains Isolated from Men Who Have Sex with Men in Québec, Canada

Affiliations

Genetic Mechanisms behind the Spread of Reduced Susceptibility to Azithromycin in Shigella Strains Isolated from Men Who Have Sex with Men in Québec, Canada

Authors

Affiliations

Abstract

Figures

Similar articles

Cited by

References

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Miscellaneous

Abstract

Figures

Similar articles

Cited by

References

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Miscellaneous