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. 2019 Jan 29;63(2):e01679-18.
doi: 10.1128/AAC.01679-18. Print 2019 Feb.

Genetic Mechanisms behind the Spread of Reduced Susceptibility to Azithromycin in Shigella Strains Isolated from Men Who Have Sex with Men in Québec, Canada

Khadidja Yousfi  1 , Christiane Gaudreau  2   3 , Pierre A Pilon  1   4 , Brigitte Lefebvre  1 , Matthew Walker  5 , Éric Fournier  1 , Florence Doualla Bell  1 , Christine Martineau  1   2 , Jean Longtin  1 , Sadjia Bekal  6   2
Affiliations

Genetic Mechanisms behind the Spread of Reduced Susceptibility to Azithromycin in Shigella Strains Isolated from Men Who Have Sex with Men in Québec, Canada

Khadidja Yousfi et al. Antimicrob Agents Chemother. .

Abstract

We analyzed 254 Shigella species isolates collected in Québec, Canada, during 2013 and 2014. Overall, 23.6% of isolates showed reduced susceptibility to azithromycin (RSA) encoded by mphA (11.6%), ermB (1.7%), or both genes (86.7%). Shigella strains with RSA were mostly isolated from men who have sex with men (68.8% or higher) from the Montreal region. A complete sequence analysis of six selected plasmids from Shigella sonnei and different serotypes of Shigella flexneri emphasized the role of IS26 in the dissemination of RSA.

Keywords: IS26 insertion sequence; Shigella spp.; plasmid diversity; reduced susceptibility to azithromycin.

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Figures

FIG 1
FIG 1
Antimicrobial resistance regions on plasmids of Shigella with determinants of reduced susceptibility to azithromycin. (a) Genes are represented by arrows and colored according to gene function. Shaded regions represent shared structures harboring mphA and ermB. Dashed line represents the nonsequenced parts. (b) Comparison of the plasmids was performed using Gview server. The sizes of the plasmids are: 82,860 bp (pSf1), 79,827 bp (pSf2), 72,298 bp (pSf3), 67,764 bp (pSf4), 89,529 bp (pSf5), and 94,328 bp (pSs1). Gene organization diagrams were drawn using Inkscape v0.91.
See this image and copyright information in PMC

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