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. 2019 Jan;26(2):1834-1847.
doi: 10.1007/s11356-018-3718-z. Epub 2018 Nov 20.

Degradation of crude oil by mixed cultures of bacteria isolated from the Qinghai-Tibet plateau and comparative analysis of metabolic mechanisms

Ruiqi Yang  1   2   3 , Gaosen Zhang  1   2 , Shiweng Li  2   4 , Faegheh Moazeni  5 , Yunshi Li  1   2   3 , Yongna Wu  1   2   3 , Wei Zhang  1   2 , Tuo Chen  6   7 , Guangxiu Liu  8   9 , Binglin Zhang  1   2 , Xiukun Wu  1   2
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Degradation of crude oil by mixed cultures of bacteria isolated from the Qinghai-Tibet plateau and comparative analysis of metabolic mechanisms

Ruiqi Yang et al. Environ Sci Pollut Res Int. 2019 Jan.

Abstract

This study investigates the biodegradation of crude oil by a mixed culture of bacteria isolated from the Qinghai-Tibet plateau using gas chromatography-mass spectrometer (GC-MS) and the gravimetric method. The results showed that a mixed culture has a stronger ability to degrade hydrocarbon than pure cultures. Once both Nocardia soli Y48 and Rhodococcus erythropolis YF28-1 (8) were present in a culture, the culture demonstrated the highest crude oil removal efficiency of almost 100% after 10 days of incubation at 20 °C. Moreover, further analysis of the degradation mechanisms used by the above strains, which revealed utilization of different n-alkane substrates, indicated the diversity of evolution and variations in different strains, as well as the importance of multiple metabolic mechanisms for alkane degradation. Therefore, it is concluded that a mixed culture of Y48 and YF28-1 (8) strains can provide a more effective method for bioremediation of hydrocarbon-contaminated soil in permafrost regions.

Keywords: Biodegradation; Crude oil; Metabolic enzyme; Mixed culture; Nocardia soli; Qinghai-Tibet plateau; Rhodococcus erythropolis.

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