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. 2018 Nov 21;18(1):107.
doi: 10.1186/s12894-018-0421-9.

Ameliorative effects of rutin against cisplatin-induced reproductive toxicity in male rats

Sarwat Jahan  1 , Asma Munawar  1 , Suhail Razak  2   3 , Sara Anam  1 , Qurat Ul Ain  1 , Hizb Ullah  1 , Tayyaba Afsar  4 , Mahmoud Abulmeaty  5 , Ali Almajwal  5
Affiliations

Ameliorative effects of rutin against cisplatin-induced reproductive toxicity in male rats

Sarwat Jahan et al. BMC Urol. .

Abstract

Background: Cisplatin (CP) or cis-diammine dichloroplatinum (II) is a platinum based standard antineoplastic drug which is used against variety of solid tumors and neoplasms. The present study aimed to evaluate the shielding effects of rutin against CP induced testicular toxicity in rats.

Methods: 28 male rats were divided into four groups. First group was given saline orally while second group received intra-peritoneal (i.p) injection of cisplatin (7 mg/kg) on day first and received saline for next 13 days. Third group received i.p injection of cisplatin at day one and treated with rutin (75 mg/kg) orally for next 13 days. Fourth group was treated with rutin orally for 13 days. Animals were sacrificed on 14th day and reproductive organs were analyzed for various parameters.

Results: Cisplatin treatment resulted in a significant decrease in daily sperm production, decrease in head length and % DNA in head, reduction of epithelial cell height, tubular diameter, reduction of the number of spermatogonia, spermatocytes and spermatids, increase in the thiobarbituric acid reactive substances (TBARS) and oxidative stress in testicular tissues, and change of the intra-testicular testosterone concentrations. Rutin co-treatment resulted in reversing cisplatin effect on DNA damage, sperm count, histological and biochemical parameters.

Conclusion: These results indicated that rutin co-treatment could ameliorate cisplatin-induced reproductive toxicity in male rats.

Keywords: Antioxidants; Cisplatin; Comet assay; Histology; Rutin; Testosterone.

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Conflict of interest statement

Ethics approval and consent to participate

This study makes use of rats and the experimental protocol for the use of animal was approved (BAS#0256) by the ethical board of Quaid-i-Azam University, Islamabad Pakistan.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Total length of chromatin dispersion in the sperm treated with (a) control, (b) Cisplatin treated, (c) Cisplatin+Rutin treated, (d) Rutin alone treated groups. 40 X. I: Intact, T: Tail, H: Head
Fig. 2
Fig. 2
Photomicrograph of seminiferous tubules of adult male rat testis (H&E, 40X) from: (a) Control group showing normal morphology of seminiferous tubule with thick germinal epithelium containing proliferating germ cells (arrow) and lumen filled with spermatids (arrow); (b) Cisplatin treated group showing disruption in spermatogenesis, increased tubular lumen (arrow) and sloughing of germinal epithelium (arrow); (c) Cisplatin+Rutin treated group showing decreased tubular diameter (arrow) and decreased interstitial space (arrow) as compared to treated group; (d) Rutin treated group showing slight increase in lumen (arrow) and interstitial spaces (arrow) as compared with control group
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