Human serum albumin templated MnO2 nanosheets are oxidase mimics for colorimetric determination of hydrogen peroxide and for enzymatic determination of glucose

Abstract

This paper reports on a colorimetric assay for H₂O₂ and glucose. It is based on the use of human serum albumin-templated MnO₂ nanosheets that possess oxidase-like activity. They are capable of oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) with oxygen to give a blue product (oxTMB) with an absorbance maximum at 652 nm. When H₂O₂ is introduced, the MnO₂ nanosheets are reduced to Mn(II) ions, and this inhibits the formation of oxTMB. Based on these findings, a colorimetric assay was established for H₂O₂ that has a 0.56 μM detection limit. If glucose is oxidized by glucose oxidase under formation of H₂O₂, the nanosheets can be used to quantify H₂O₂ and thereby to sense glucose. Response is linear in the 0.5 μM to 50 μM glucose concentration range, and the detection limit is 0.32 μM. The method was applied to the determination of glucose in spiked serum samples and gave satisficatory results. Graphical abstract Human serum albumin (HSA) is used as a template for the synthesis of MnO₂ nanosheet. These possess oxidase mimicking activity. H₂O₂ can reduce the nanosheets. The effect is exploited in colorimetric assays for H₂O₂ and glucose using tetramethylbenzidine (TMB) as a chromogenic substrate.

Keywords: 3,3 ́,5,5 ́-Tetramethylbenzidine; Colorimetric assay; Glucose oxidase; HSA; Human serum; Optical sensor.