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. 1988 Sep 13;112(2):201-5.
doi: 10.1016/0022-1759(88)90358-4.

Malaria sporozoite penetration. A new approach by double staining

L Rénia  1 , F Miltgen, Y Charoenvit, T Ponnudurai, J P Verhave, W E Collins, D Mazier
Affiliations

Malaria sporozoite penetration. A new approach by double staining

L Rénia et al. J Immunol Methods. .

Abstract

To determine, whether a sporozoite is outside the hepatocyte membrane or internalized, a double staining test was carried out using, successively, antibody labeled with peroxidase and fluorescein. This test permits the quantification of sporozoite entry and outline sporozoite-hepatocyte interactions.

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