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Case Reports
. 2019 Jan 8;81(1):91-94.
doi: 10.1292/jvms.18-0253. Epub 2018 Nov 26.

X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites

Shiori Gamo  1 , Teruaki Tozaki  1   2 , Hironaga Kakoi  2 , Kei-Ichi Hirota  2 , Kotono Nakamura  1 , Noriko Nishii  1 , Julio Alumunia  1 , Masaki Takasu  1   3
Affiliations
Case Reports

X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites

Shiori Gamo et al. J Vet Med Sci. .

Abstract

A routine parentage test as part of a conservation program for Kiso horses identified a possible sex chromosome anomaly in a 7 months-old filly because of an aberrant result using LEX3, an X-linked marker. We then analyzed X-linked markers (LEX26, TKY38, and TKY270), Y-linked markers (Eca.YH12, Eca.YM2, Eca.YA16, and the sex-determining region Y gene), and an X/Y marker (Amelogenin gene). This analysis demonstrated that the filly had not inherited an X chromosome from her sire. A karyotyping analysis confirmed that the filly was 63,XO. As it was suspected that the horse would be sterile, we avoided using the horse as a broodmare; the information should also serve to prevent unnecessary conflict between owners transferring and receiving the horse.

Keywords: Kiso horse; XO monosomy; parentage test.

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Figures

Fig. 1.
Fig. 1.
Appearance of the 63,XO Kiso horse at 7 months of age. No abnormality was observed in her appearance.
Fig. 2.
Fig. 2.
R-banded karyotype of the filly. The analysis revealed the presence of a single X chromosome.
See this image and copyright information in PMC

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