Thyroid hormones mediate metabolic rate and oxidative, anti-oxidative balance at different temperatures in Mongolian gerbils (Meriones unguiculatus)

Abstract

Oxidative damage is a potential physiological cost of thermoregulation during seasonal adjustments to air temperature (T_a) in small mammals. Here, we hypothesized that T_a affects serum thyroid hormone levels and these hormones can mediate the changes in metabolic rate and oxidative damage. Mongolian gerbils (Meriones unguiculatus) were acclimated at different T_as (5 °C, 23 °C and 37 °C) for 3 weeks. Serum tri-iodothyronine (T3) levels increased at 5 °C but decreased at 37 °C compared to the control (23 °C). Protein carbonyls increased in liver at 37 °C compared with control, however, lipid damage (malonaldehyde, MDA) in both serum and liver was unrelated to T_a. After the effects of different T_as on thyroid hormone levels and oxidative damage markers were determined, we further investigate whether thyroid hormones mediated metabolic rate and oxidative damage. Another set of gerbils received 0.0036% L-thyroxin (hyperthyroid), 0.04% Methylimazol (hypothyroid) or water (control). Hypothyroid group showed a 34% reduction in resting metabolic rate (RMR) also 42% and 26% increases in MDA and liver protein carbonyl respectively, whereas hyperthyroid group had higher RMR, liver mass and superoxide dismutase (SOD) compared to control. Serum T3 or T3/T4 levels were correlated positively with RMR, liver mass, and SOD, but negatively with MDA and uncoupling protein 2 (UCP2). We concluded that high T_a induced hypothyroidism, decreased RMR and increased oxidative damage, whereas low T_a induced hyperthyroidism, increased RMR and unchanged oxidative damage. These data supported our hypothesis that thyroid hormones can be a cue to mediate metabolic rate and different aspects of oxidative and antioxidant activities at different T_as.

Keywords: Metabolic rate; Mongolian gerbils (Meriones unguiculatus); Oxidative damage; Thyroid hormones; Uncoupling protein 2 (UCP2).