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Review
. 2018 Dec;15(12):1011-1019.
doi: 10.1038/s41592-018-0211-z. Epub 2018 Nov 26.

Faster, sharper, and deeper: structured illumination microscopy for biological imaging

Yicong Wu  1 , Hari Shroff  2
Affiliations
Review

Faster, sharper, and deeper: structured illumination microscopy for biological imaging

Yicong Wu et al. Nat Methods. 2018 Dec.

Erratum in

Abstract

Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.

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