Multimodal Cancer Theranosis Using Hyaluronate-Conjugated Molybdenum Disulfide

Abstract

Among various 2D nanomaterials, molybdenum disulfide (MoS₂ ) exhibits unique visible photoluminescence with high absorption at the near-infrared (NIR) range. Despite these optical properties, the efforts to use MoS₂ nanomaterials for optical imaging and photothermal therapy are hampered by their instability and low intracellular delivery efficiency. Multifunctional MoS₂ conjugated with hyaluronate (HA) for cancer theranosis is reported herein. HA facilitates the delivery of MoS₂ to tumor cells by the HA-receptor mediated endocytosis. In BALB/c nude mice inoculated with a colorectal cancer cell line of HCT116, HA-MoS₂ conjugates appear to be accumulated in the primary tumor at a content more than that in the liver and kidney. The disulfide bonding between MoS₂ and thiolated HA seems to degrade in the cytoplasm, releasing MoS₂ sheets in stacks and enhancing luminescence efficiency. The HA-MoS₂ conjugates are readily detected via photoacoustic imaging as well as upconversion and downconversion fluorescence imaging. With NIR light illumination, HA-MoS₂ conjugates enable highly effective photothermal tumor ablation. All these results confirm the promising potential of HA-MoS₂ conjugates for cancer theranosis.

Keywords: hyaluronate; intracellular reduction; molybdenum disulfide; multifunctional theranosis; tumor targeting.