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. 2019 Mar;33(3):e22826.
doi: 10.1002/jcla.22826. Epub 2018 Nov 28.

Association of the study between LncRNA-H19 gene polymorphisms with the risk of breast cancer

Safa Abdollahzadeh  1 , Saeid Ghorbian  1
Affiliations

Association of the study between LncRNA-H19 gene polymorphisms with the risk of breast cancer

Safa Abdollahzadeh et al. J Clin Lab Anal. 2019 Mar.

Abstract

Background: The H19 is a maternally expressed imprinted gene transcribing a long noncoding RNA (lncRNA), which has previously been reported to be involved in tumorigenesis and cancer progression. The aim of this study was to evaluate the associations between two lncRNA-H19 (rs3741219 T>C and rs217727 C>T) gene polymorphisms with the risk of breast cancer (BC).

Methods: In a case-control investigation, we evaluated 150 BC patients and 100 cancer-free subjects in East Azerbaijan Province of Iran. To assess two gene polymorphisms, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used.

Results: The genotype frequencies of two lncRNA-H19 (rs217727 C>T and rs3741219 T>C) gene polymorphisms TT + TC/CC and CC + CT/TT have not shown a statistically significant association with the risk of BC (P = 0.065; OR = 0.967; 95% CI, 0.938-0.996) and (P = 0.510; OR = 1.583; 95% CI, 0.399-6.726), respectively. In addition, our findings revealed a significant differences in allele frequencies in lncRNA-H19 rs217727 C>T polymorphism between groups (P = 0.033; OR = 1.985; 95% CI, 1.048-3.761).

Conclusion: Our findings suggested that rs217727 C>T polymorphism may be involved in the pathogenesis of BC, whereas rs3741219 T>C variation may not be involved in the genetic background of BC in Iranian.

Keywords: LncRNA; LncRNA-H19; H19 gene; breast cancer.

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Figures

Figure 1
Figure 1
Gel electrophoresis of the PCR‐RFLP products from of lncRNA‐H19 (rs3741219) gene on 3% agarose gel electrophoresis. Lanes 1 and 3: TC genotype (342, 301, 92, and 41 bp); Lane 2: CC genotype (301, 92, and 41 bp); Lane 4: TT genotype (342 and 92 bp); M: DNA size marker 100 bp
Figure 2
Figure 2
Gel electrophoresis of the PCR‐RFLP products from of lncRNA‐H19 (rs217727) gene on 3% agarose gel electrophoresis. Lane 1: CT genotype (247, 221, and 26 bp); Lane 2: CC genotype (221 and 26 bp); Lane 3: TT genotype (247 bp); M: DNA size marker 100 bp
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