Effect of Nutrient Media on Antibacterial Activity of Silver Nanoparticles Synthesized Using Neolamarckia cadamba

Abstract

Silver nanoparticles synthesized using leaf and fruit extracts which were centrifuged at different speeds (rev/min) were screened for anti-bacterial activity by using four human pathogens namely E. coli, P. aeroginosa (Gram negative) and S. aureus, B. subtilis (Gram positive). The silver nanoparticles showed good anti-bacterial activity against gram-positive as well as gram-negative bacteria. Nonetheless, silver nanoparticles synthesized using leaf extract has shown pre-dominant anti-bacterial activity rather than fruit extract. When comparing the antibacterial activity by using different media, nutrient agar and Luria Bertini agar have shown better results than Mueller Hinton agar for the same set of bacteria as well as nanoparticles. Silver nanoparticles showed different activity in different media which can be extrapolated to the way in which anti-microbial agents act in different environment relating it to its functioning in different human bodies.