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. 1988 Oct;170(10):4969-71.
doi: 10.1128/jb.170.10.4969-4971.1988.

Availability of porphobilinogen controls appearance of porphobilinogen deaminase activity in Escherichia coli K-12

H Umanoff  1 , C S Russell, S D Cosloy
Affiliations

Availability of porphobilinogen controls appearance of porphobilinogen deaminase activity in Escherichia coli K-12

H Umanoff et al. J Bacteriol. 1988 Oct.

Abstract

A hemin-permeable hemB mutant had no 5-aminolevulinate dehydratase (ALA D) and extremely low porphobilinogen deaminase (PBG D) activity. When the structural gene for hemB was introduced into this strain on a single-copy plasmid, both activities were observed. When the mutant was grown on PBG, normal PBG D activity was observed. Moreover, a hemA mutant had little or no PBG D activity unless it was grown on ALA or PBG. Neither hemin nor PBG affected the level of PBG D protein produced from in vitro transcription and translation of a plasmid harboring the hemC gene as an insert. We conclude that, in Escherichia coli, PBG availability controls the activity of PBG D at some posttranscriptional level.

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References

    1. J Bacteriol. 1968 Nov;96(5):1882-4 - PubMed
    1. Annu Rev Genet. 1973;7:267-87 - PubMed
    1. J Biol Chem. 1980 Mar 10;255(5):1993-9 - PubMed
    1. Microbiol Rev. 1983 Jun;47(2):180-230 - PubMed
    1. J Bacteriol. 1985 May;162(2):777-83 - PubMed

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