Salicylic acid-altering Arabidopsis plant response to cadmium exposure: Underlying mechanisms affecting antioxidation and photosynthesis-related processes

Abstract

Previous studies have demonstrated that the genetic modification of basal salicylic acid (SA) level changed Arabidopsis plant response to cadmium (Cd) stress, but the mechanisms remain evaluated. In this study, Arabidopsis wild type (WT) and its SA-reducing transgenic line nahG (naphthalene hydroxylase G), SA-accumulating mutant snc1 (suppressor of nonexpressor of PR gene, constitutive 1) were exposed to 50 μM Cd²⁺ for 48 h or 7 d (just for assessing plant growth). The Cd treatment increased the expression levels of SA biosynthesis-related genes leading to enhanced SA accumulations in plant leaves, which was further confirmed by the expression patterns of SA marker genes. Cadmium accumulation was much higher in the Cd-exposed roots than in leaves, but was not affected by SA levels. Exposure to Cd inhibited plant growth of both aerial parts and roots, to a greater degree in snc1, and a lesser extent in nahG as compared with WT. Although Cd treatment increased plant antioxidative capacity, oxidative damage happened, especially to snc1 plants. Photoinhibition occurred in Cd-stressed plants leading to a decrease in photosynthetic activity, with a greater degree in snc1, while a lesser in nahG, as indicated by the changes of several key photosynthetic parameters. We comprehensively analyzed the expression profiles of photosynthesis-related genes, and observed a positive correlation between Cd tolerance and gene expression levels, wherein the transcription levels of two electron transport-related genes and two amylase-encoding genes were all up-regulated in nahG plants after Cd treatment, implying a significance of the related processes in this genotype against Cd stress.

Keywords: Antioxidative defense; Arabidopsis thaliana; Cadmium stress; Photosynthesis; Salicylic acid.