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. 2018 Oct;45(5):341-346.
doi: 10.1159/000486606. Epub 2018 Mar 9.

Evaluation of the New Lateral Flow Card MDmulticard® Basic Extended Phenotype in Routine Clinical Practice

Beate Mayer  1 , Julia Müller  2 , María-José Candela-García  3 , Annie-Claude Manteau  4 , Christof Weinstock  2 , Axel Pruß  1
Affiliations

Evaluation of the New Lateral Flow Card MDmulticard® Basic Extended Phenotype in Routine Clinical Practice

Beate Mayer et al. Transfus Med Hemother. 2018 Oct.

Abstract

Background: Transfusion emergencies and critical situations require specifically designed devices to simplify and optimize the standard procedures. In addition, matching antigens over and above ABO-Rh-K would be beneficial.

Methods: Routine blood samples were collected in four immunohematology centers and tested with the new MDmulticard Basic Extended Phenotype for the simultaneous detection of the Duffy, Kidd, and Ss antigens, according to the principle of the lateral flow. Results were compared with those obtained using routine serology methods. Discrepancies were analyzed by molecular techniques/genotyping.

Results: 310 samples were tested (167 donors; 75 patients; 28 subjects with positive direct antiglobulin test (DAT); 15 newborns; 25 previously transfused patients). The 285 samples with non-mixed-field reaction yielded 1,710 antigen results with 8 discrepancies (0.47%) six of which in DAT-positive subjects: three false-positive (Fya) for MDmulticard, and two false-positive (Fya) plus three false-negative (Fyb) for the reference methods (MDmulticard PPA for donors/patients/newborns: 99.82%; negative percent agreement: 100%; sensitivity: 100%; specificity: 99.39%, positive predictive value: 99.75%; negative predictive value: 100%). The MDmulticard detected mixed-field in 15 antigen reactions from 13 transfused patients, undetected by the comparative method, with the opposite result in 8 antigens (5 patients).

Conclusion: The MDmulticard Basic Extended Phenotype met the criteria prescribed for the testing of donor, patient, DAT-positive, and newborn samples in transfusion laboratory routine.

Keywords: Duffy antigen; Kidd antigen; Lateral flow technology; MDmulticard; Ss antigens.

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Figures

Fig. 1
Fig. 1
Positive reactions as they occur in the MDmulticard Basic Extended Phenotype: A) strong reaction 3+ (arrow-shaped band) in Fyb; B) weak reaction 1+ (weak band) in Fya; and C) mixed-field reaction (half band) in Fya and S.
See this image and copyright information in PMC

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