Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Dec 2:24:8716-8721.
doi: 10.12659/MSM.910931.

Analysis of Immunologic Function Changes in Lichen Planus After Clinical Treatment

Hua Qian  1 , Liyan Jiao  1 , Zhixia Fan  1 , Litao Wang  1 , Baoguo Liu  1 , Guoying Miao  1
Affiliations

Analysis of Immunologic Function Changes in Lichen Planus After Clinical Treatment

Hua Qian et al. Med Sci Monit. .

Abstract

BACKGROUND Lichen planus (LP) is a common chronic superficial skin lesion that causes chronic or sub-acute inflammatory disorders. LP can affect the oral cavity, skin, mucous membrane, and other body parts, and features include repeat attacks and long duration, leading to lower quality of life. This study aimed to analyze the changes of immunologic function before and after treatment of LP. MATERIAL AND METHODS Thirty cutaneous LP patients were selected. Peripheral blood was collected in the morning before and after treatment. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient method. Flow cytometry was used to detect T cell subpopulation CD4⁺ T cells and CD8⁺ T to calculate CD4⁺ T/CD8⁺ T ratio. Enzyme-linked immunosorbent assay (ELISA) was adopted to detect the helper T-cell (Th) factor IL-2, IFN-γ, IL-4, IL-6, IL-17, and IL-22 levels. RESULTS Compared with before treatment, the expressions of CD4⁺ T cells and CD8⁺ T cells were decreased, while the proportion of CD4⁺ T/CD8⁺ T were significantly elevated after treatment. IL-2 and IFN-γ secretion were markedly increased, whereas IL-4, IL-6, IL-17, and IL-22 were significantly reduced after treatment (P<0.05). CONCLUSIONS LP treatment reduces the distribution of CD4⁺ T cells and CD8⁺ T cells, and promotes the changes of Th1, Th2, and Th17 cytokines secretion.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest

None.

Figures

Figure 1
Figure 1
CD4+ T cell detection in lichen planus patients after treatment.
Figure 2
Figure 2
CD4+ T cell population changes in lichen planus patients after treatment. * P<0.05, compared with before treatment.
Figure 3
Figure 3
CD8+ T cell detection in lichen planus patients after treatment.
Figure 4
Figure 4
CD8+ T cell population changes in lichen planus patients after treatment. * P<0.05, compared with before treatment.
Figure 5
Figure 5
CD4+ T/CD8+ T cell ratio changes in lichen planus patients after treatment. * P<0.05, compared with before treatment.
Figure 6
Figure 6
IL-2 and IFN-γ expressions in lichen planus patients after treatment. * P<0.05, compared with before treatment.
Figure 7
Figure 7
IL-4 and IL-6 expressions in lichen planus patients after treatment. * P<0.05, compared with before treatment.
Figure 8
Figure 8
IL-17 and IL-22 expressions in lichen planus patients after treatment. * P<0.05, compared with before treatment.
See this image and copyright information in PMC

References

    1. Wang YP, Wu YC, Cheng SJ, et al. High frequencies of vitamin B 12, and folic acid deficiencies and gastric parietal cell antibody positivity in oral submucous fibrosis patients. J Formosan Med Assoc. 2015;114(9):813–19. - PubMed
    1. Radwan-Oczko M, Zwyrtek E, Owczarek JE, et al. Psychopathological profile and quality of life of patients with oral lichen planus. J Appl Oral Sci. 2018;26:e20170146. - PMC - PubMed
    1. Boccellino M, Di Stasio D, Romano A, et al. Lichen planus: molecular pathway and clinical implications in oral disorders. J Biol Regul Homeost Agents. 2018;32:135–38. - PubMed
    1. Wang TY, Chiu YW, Chen YT, et al. Malignant transformation of Taiwanese patients with oral leukoplakia: A nationwide population-based retrospective cohort study. J Formosan Med Assoc. 2018;117(5):374–80. - PubMed
    1. Mirza S, Rehman N, Alrahla A, et al. Efficacy of photodynamic therapy and low level laser therapy against steroid therapy in the treatment of erosive-atrophic oral lichen planus. Photodiagnosis Photodyn Ther. 2018;21:404–8. - PubMed

MeSH terms