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. 2018 Nov;25(7):1308-1321.
doi: 10.1016/j.sjbs.2016.02.017. Epub 2016 Feb 15.

Enhanced butanol production by optimization of medium parameters using Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani  1   2 , Hafiza Shukor  3 , Peyman Abdeshahian  3 , Mohd Sahaid Kalil  3 , Wan Mohtar Wan Yusoff  1 , Aidil Abdul Hamid  1
Affiliations

Enhanced butanol production by optimization of medium parameters using Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani et al. Saudi J Biol Sci. 2018 Nov.

Abstract

A new isolate of the solvent-producing Clostridium acetobutylicum YM1 was used to produce butanol in batch culture fermentation. The effects of glucose concentration, butyric acid addition and C/N ratio were studied conventionally (one-factor-at-a-time). Moreover, the interactions between glucose concentration, butyric acid addition and C/N ratio were further investigated to optimize butanol production using response surface methodology (RSM). A central composite design was applied, and a polynomial regression model with a quadratic term was used to analyze the experimental data using analysis of variance (ANOVA). ANOVA revealed that the model was highly significant (p < 0.0001) and the effects of the glucose and butyric acid concentrations on butanol production were significant. The model validation experiment showed 13.82 g/L butanol was produced under optimum conditions. Scale up fermentation in optimized medium resulted in 17 g/L of butanol and 21.71 g/L of ABE. The experimental data of scale up in 5 L bioreactor and flask scale were fitted to kinetic mathematical models published in the literature to estimate the kinetic parameters of the fermentation. The models used gave the best fit for butanol production, biomass and glucose consumption for both flask scale and bioreactor scale up.

Keywords: Butanol; Clostridium acetobutylicum YM1; Fermentation; Kinetics; Optimization; Scale-up.

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Figures

Figure 1
Figure 1
Conversion of butyrate to butanol via two suggested mechanisms: (a) CoAT pathway and (b) butyrate kinase pathway. CoAT; CoA transferase, BADH; butylaldehyde dehydrogenase, BDH; butanol dehydrogenase, BK; butyrate kinase, PTB; phosphotransbutyrylase.
Figure 2
Figure 2
Response surface plot of butanol production from C. acetobutylicum YM1 showing the interaction between glucose concentration and C/N ratio (a), glucose concentration and butyric acid concentration (b), and butyric acid concentration and C/N ratio (c).
Figure 3
Figure 3
Effect of butanol on growth of C. acetobutylicum YM1.
Figure 4
Figure 4
Butanol fermentation in optimized fermentation medium by batch culture of C. acetobutylicum YM1; (a) scale up fermentor experiment and (b) flask scale experiment.
Figure 5
Figure 5
Fitting of the experimental data to the model describing butanol production over time in optimized fermentation medium; (a) bioreactor scale up experiment and (b) flask scale experiment.
Figure 6
Figure 6
Fitting of the experimental data to the model describing biomass production over time in optimized fermentation medium; (a) bioreactor scale up experiment and (b) flask scale experiment.
Figure 7
Figure 7
Fitting of the experimental data to the model describing glucose consumption over time in optimized fermentation medium; (a) bioreactor scale up experiment and (b) flask scale experiment.
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