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. 2018 Nov;25(7):1420-1428.
doi: 10.1016/j.sjbs.2015.11.009. Epub 2015 Nov 26.

Identification of bioactive phytochemical from two Punica species using GC-MS and estimation of antioxidant activity of seed extracts

Asma A Al-Huqail  1 , Gehan A Elgaaly  1 , Mohamed M Ibrahim  1   2
Affiliations

Identification of bioactive phytochemical from two Punica species using GC-MS and estimation of antioxidant activity of seed extracts

Asma A Al-Huqail et al. Saudi J Biol Sci. 2018 Nov.

Abstract

Punica species are medicinally important plants belonging to the family Lythraceae. The pomegranate is widely reported to exhibit antiviral, antioxidant, anticancer, anti-proliferative activities. In the present study the ethanolic extract of the peel seeds of two species of Punica (Punica granatum and Punica protopunica) were subjected to GC-MS analysis. Twenty-one and 14 compounds were identified in P. granatum and P. protopunica peel seeds, respectively. The main chemical constituents in P. granatum-peel seeds were propanoic acid, benzenedicarboxylic acid, methoxypropionic acid and methyl amine. The corresponding constituents of P. protopunica peel seeds were benzenedicarboxylic acid, benzoic acid and propanoic acid. Moreover, the antioxidant effects of the aqueous ethanolic extracts were estimated in vitro. The two tested extracts contained significantly different phenolic and total flavonoid contents in P. granatum than in P. protopunica. Different in vitro methods of antioxidant activity determination produced varying results. In malondialdehyde (MDA), hydrogen peroxide (H2O2) scavenging and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, the two peel seed extracts exhibited very high antioxidant activities, with higher activity observed for the P. granatum extract.

Keywords: Antioxidants; Bioactive compounds; GC–MS analysis; Punica species.

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Figures

Figure 1
Figure 1
Antioxidant capabilities [TBARS, Thiobarbituric acid reactive species method; Sc.A. of (NO), nitric oxide (NO) scavenging activity and Sc.A. of (DPPH), radical scavenging effects of the extract on DPPH free radical] of Punica granatum (A) and Punica protopunica extracts. Data are presented as the mean ± SE (n = 3). *Significantly correlated (p ⩽ 0.05).
Figure 2
Figure 2
GC–MS chromatogram of ethanolic extract of Punica granatum seeds.
Figure 3
Figure 3
GC–MS chromatogram of ethanolic extract of Punica protopunica seeds.
See this image and copyright information in PMC

References

    1. Abdel Moneim A.E., Mohamed A.D., Al-Quraishy S. Studies on the effect of pomegranate (Punica granatum) juice and peel on liver and kidney in adult male rats. J. Med. Plants Res. 2011;5(20):5083–5088.
    1. Adhami V.M., Mukhtar H. Polyphenols from green tea and pomegranate for prevention of prostate cancer. Free Radical Res. 2006;40(10):1095–1104. - PubMed
    1. Adhami V.M., Mukhtar H. Anti-oxidants from green tea and pomegranate for chemoprevention of prostate cancer. Mol. Biotechnol. 2007;37:52–57. - PubMed
    1. Ajaikumar K.B., Asheef M., Babu B.H., Padikkala J. The inhibition of gastric mucosal injury by Punica granatum L. (pomegranate) methanolic extract. J. Ethnopharmacol. 2005;96:171–176. - PubMed
    1. Araceli S., Camen R.M., Guillermo R.S. Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside. Eur. J. Pharmacol. 2003;461(2003):53–61. - PubMed

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