High-throughput sequencing of hair follicle development-related micrornas in cashmere goat at various fetal periods

Abstract

Inner Mongolia cashmere goat marks a precious gerplasm genetic resource due to its excellent cashmere traits. Therefore, it is of crucial importance to investigate the cashmere development mechanism of cashmere goat and to search for the important cashmere growth-related candidate genes. Fetal skin samples at 10 different periods of cashmere goat were collected in this research. Moreover, high-throughput sequencing was conducted on RNA samples from side skin of cashmere goat fetuses collected at three critical periods of skin hair follicle initiation, growth and development (namely, 45, 55 and 65 days) after balanced mix in line with the previous research results. Meanwhile, 3 samples at corresponding periods were used as the biological duplications. Data regarding microRNA and mRNA expression in skin and hair follicles of cashmere goats at various fetal periods were obtained using the high-throughput sequencing method. The results indicated that microRNAs in the oar-let-7 and oar-miR-200 families in 55 days and 66 days of pregnancy samples had been notably up-regulated relative to those in 45 days of pregnancy samples. This revealed that they might be the critical microRNAs in hair follicle development.

Keywords: Cashmere goat; Cyclic variation; Hair follicle; High-throughput sequencing; MicroRNA.

Fig. 1

Technical route.

Fig. 2

Electrophoresis detection.

Fig. 3

cDNA library construction.

Fig. 4

sRNA-seq workflow.

Fig. 5

Clean reads sequencing quality analysis (Cashmere_goat_45_fastqc).

Fig. 6

Clean reads sequencing quality analysis (Cashmere_goat_55_fastqc).

Fig. 7

Clean reads sequencing quality analysis (Cashmere_goat_65_fastqc).

Fig. 8

Effective length statistics (Cashmere_goat_45_fastqc).

Fig. 9

Effective length statistics (Cashmere_goat_55_fastqc).

Fig. 10

Effective length statistics (Cashmere_goat_65_fastqc).

Fig. 11

Result presentation (Cashmere_goat_45_fastqc), Sample of Day 45 cashmere goat PCR duplication level〉94.53%.

Fig. 12

Result presentation (Cashmere_goat_55_fastqc), Sample of Day 55 cashmere goat PCR duplication level >95.87%.

Fig. 13

Result presentation (Cashmere_goat_65_fastqc), Sample of Day 65 cashmere goat PCR duplication level >93.82%.

Fig. 14

Cashmere_goat_45 Reads distribution across reference Genomic Regions.

Fig. 15

Cashmere_goat_55 Reads distribution across reference Genomic Regions.

Fig. 16

Cashmere_goat_65 Reads distribution across reference Genomic Regions.

Fig. 17

Goat_45 Rfam classification.

Fig. 18

Goat_55 Rfam classification.

Fig. 19

Goat_65 Rfam classification.

Fig. 20

DEG workflow.

Fig. 21

Sample correlation analysis.