Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019:1885:347-359.
doi: 10.1007/978-1-4939-8889-1_23.

Noninvasive Antenatal Screening for Fetal RHD in RhD Negative Women to Guide Targeted Anti-D Prophylaxis

Frederik Banch Clausen  1 , Klaus Rieneck  2 , Grethe Risum Krog  2 , Birgitte Suhr Bundgaard  2 , Morten Hanefeld Dziegiel  2   3
Affiliations

Noninvasive Antenatal Screening for Fetal RHD in RhD Negative Women to Guide Targeted Anti-D Prophylaxis

Frederik Banch Clausen et al. Methods Mol Biol. 2019.

Abstract

RhD negative pregnant women who carry an RhD positive fetus are at risk of immunization against the D antigen, which may result in hemolytic disease of the fetus and the newborn. Predicting the fetal RhD status by noninvasive antenatal screening for the fetal RhD gene (RHD) can guide targeted use of antenatal anti-D prophylaxis.Cell-free fetal DNA is extracted from maternal plasma from RhD negative pregnant women at a gestational age of 25 weeks. A real-time PCR-based detection of two RHD exons enables reliable prediction of the fetal RhD status to determine the administration of antenatal prophylaxis, as well as postnatal prophylaxis.

Keywords: Antenatal prophylaxis; Anti-D; Cell-free fetal DNA; Hemolytic disease of the fetus and newborn; Noninvasive RHD typing; Real-time PCR.

PubMed Disclaimer

MeSH terms

LinkOut - more resources