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. 2019 Feb;46(1):705-718.
doi: 10.1007/s11033-018-4526-8. Epub 2018 Dec 1.

Isolation and characterization of three skeletal troponin genes and association with growth-related traits in Exopalaemon carinicauda

Jiajia Wang  1   2 , Qianqian Ge  1   2 , Jitao Li  1   2 , Zhao Chen  1   2 , Jian Li  3   4
Affiliations

Isolation and characterization of three skeletal troponin genes and association with growth-related traits in Exopalaemon carinicauda

Jiajia Wang et al. Mol Biol Rep. 2019 Feb.

Abstract

Growth is among the most important traits for animal breeding. Muscle growth is controlled by different cellular and molecular pathways and environments, and it also relies heavily on high-quality muscle contractions. The troponin complex, composed of troponin T (TnT), troponin C (TnC) and troponin I (TnI), plays a vital role in the regulation of muscle contraction. In this study, the cDNA of EcTnT, EcTnC and EcTnI of the ridgetail white prawn Exopalaemon carinicauda were cloned and characterized. The full length cDNA of EcTnT, EcTnC and EcTnI were 1 373 bp, 692 bp, and 1 475 bp, encoding a protein of 385, 150 and 193 amino acid residues, respectively. The expression of all genes was predominantly detected in abdominal muscle, while extremely lesser expressed in gill and hepatopancreas. Higher expression level of EcTnI was observed in heavier shrimp of the same age during different developmental stages, excepted for 120 days. Eleven single nucleotide polymorphisms (SNPs) were revealed in the three skeletal troponin genes, and only c.TnI66 A>G from EcTnI was significantly associated with both body weight and body length (P < 0.05). In summary, the result of this study suggested that EcTnI is growth-related gene of the troponin complex gene and the presence of SNP suggests that it could be a candidate gene for shrimp genetic improvement research.

Keywords: Exopalaemon carinicauda; Gene expression; Growth-related; SNP; Troponin.

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References

    1. Xu W, Xie J, Shi H, Li C (2010) Hematodinium infections in cultured ridgetail white prawns, Exopalaemon carinicauda, in eastern China. Aquaculture 300(1):25–31 - DOI
    1. Herzberg O, James MN (1985) Structure of the calcium regulatory muscle protein troponin-C at 2.8 A resolution. Nature 313(6004):653–659 - PubMed - DOI
    1. Narita A, Yasunaga T, Ishikawa T, Mayanagi K, Wakabayashi T (2001) Ca2+-induced switching of troponin and tropomyosin on actin filaments as revealed by electron cryo-microscopy 1. J Mol Biol 308(2):241–261 - PubMed - DOI
    1. Chen H, Wang D, Xu G, Zheng C (2012) Cloning, structural analysis and expression of cardiac troponin C (TNNC1) gene in goat. Afr J Biotechnol 11(16):3903–3910
    1. Jensen D, Reynolds N, Yang YP, Shakya S, Wang ZQ, Stuehr DJ, Wei CC (2015) The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca2+-induced hydrophobicity and alter the interaction with Orai1: a spectroscopic, thermodynamic and kinetic study. BMC Biochem 16(1):1–18 - DOI

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