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. 2018 Oct;50(3):148-151.
doi: 10.5152/eurasianjmed.2018.17123.

Effect of Nigella sativa Ethanol Extract on the Nitric Oxide Content and Renal Arteriole Diameter of a Pre-eclampsia Mouse Model

Ni Made Dwi Purnamayanti  1 , Siti Candra Windu  2 , Sri Poeranto  3
Affiliations

Effect of Nigella sativa Ethanol Extract on the Nitric Oxide Content and Renal Arteriole Diameter of a Pre-eclampsia Mouse Model

Ni Made Dwi Purnamayanti et al. Eurasian J Med. 2018 Oct.

Abstract

Objective: The aim of this study was to investigate the effect of Nigella sativa ethanol extract on nitric oxide (NO) levels and renal arteriole diameter of a pre-eclampsia mouse model.

Materials and methods: This experimental study was conducted using a post-test only control group design. Thirty BALB/c mice were divided into six groups: one negative control (normal pregnant mice), one positive control (pre-eclampsia model), and four groups of pre-eclampsia mice treated with varying doses of N. sativa (500 mg/kg body weight (BW)/day, 1000 mg/kg BW/day, 1500 mg/kg BW/day, and 2000 mg/kg BW/day). Ethanol extract of N. sativa was given for 5 days. Data were analyzed using analysis of variance.

Results: We detected significant differences in NO levels between the pre-eclampsia mouse model and those given the ethanol extract. The latter had NO levels of 85.77±4.47 μM (500 mg/kg BW/day), 189.04±6.01 μM (1000 mg/kg BW/day), 226.56±2.13 μM (1500 mg/BW/day), and 207.98±4.74 μM (2000 mg/kg BW/day). The mean renal arteriole diameter showed significant differences among the treatment groups with N. sativa doses of 1000, 1500, and 2000 mg (15.15±2.21b μm, 16.35±2.52b μm, and 15.76±3.03b μm, respectively).

Conclusion: N. sativa ethanol extract treatment increases NO levels and enlarges renal arteriole diameter of a pre-eclampsia mouse model in a dose-dependent manner.

Keywords: Nigella sativa; Nitric oxide; pre-eclampsia; renal arteriole diameter.

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Conflict of interest statement

Conflict of Interest: Authors have no conflict of interest to declare.

Figures

Figure 1
Figure 1
The effect of N. sativa ethanol extract on NO levels of pre-eclampsia mice model
Figure 2
Figure 2
The effect of N. sativa ethanol extract on renal arteriole diameter of pre-eclampsia mice model
See this image and copyright information in PMC

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