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. 2020 Jan;69(1):191-193.
doi: 10.1136/gutjnl-2018-317726. Epub 2018 Dec 5.

Increased levels of systemic LPS-positive bacterial extracellular vesicles in patients with intestinal barrier dysfunction

Joeri Tulkens  1   2 , Glenn Vergauwen  1   2   3 , Jan Van Deun  1   2 , Edward Geeurickx  1   2 , Bert Dhondt  1   2   4 , Lien Lippens  1   2   5 , Marie-Angélique De Scheerder  6 , Ilkka Miinalainen  7 , Pekka Rappu  8 , Bruno G De Geest  2   9 , Katrien Vandecasteele  2   10 , Debby Laukens  11   12 , Linos Vandekerckhove  6 , Hannelore Denys  2   5 , Jo Vandesompele  2   13 , Olivier De Wever  1   2 , An Hendrix  1   2
Affiliations

Increased levels of systemic LPS-positive bacterial extracellular vesicles in patients with intestinal barrier dysfunction

Joeri Tulkens et al. Gut. 2020 Jan.
No abstract available

Keywords: HIV/AIDS; IBD; cancer; endotoxin; intestinal barrier function.

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Conflict of interest statement

Competing interests: None declared.

Figures

Figure 1
Figure 1
Quantitative and qualitative assessment of lipopolysaccharide (LPS)-positive bacterial extracellular vesicles (EV) isolated from plasma of patients with intestinal barrier dysfunction. (A–C) Box and whisker plots of LPS activity levels, (D) z-score transformation of Toll-like receptor 4 (TLR4) agonistic activity levels, and (E) immunoelectron microscopy analysis (scale bar=200 nm) of systemic bacterial EV in patients diagnosed with HIV, IBD and cancer therapy-induced intestinal mucositis compared with respective controls (each block represents one individual). (F) Correlation analysis between plasma zonulin and bacterial EV-associated LPS activity levels (Spearman’s r=0.4241, p=2.45×10−2). EU, endotoxin unit.
Figure 2
Figure 2
Intestinal barrier dysfunction opens the door for bacterial EV-associated LPS and other LPS products to enter the systemic circulation and induce immune activation. EV, extracellular vesicles; LPS, lipopolysaccharide.
See this image and copyright information in PMC

References

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