Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jan;57(1):20-23.
doi: 10.5414/CP203294.

Sputum induction for assessment of biomarkers in early respiratory drug development

Robert Lins, Haiko Pillu, Lien Gheyle, Annick van Riel, Muriel Lins, Frederic Vanhoutte

Sputum induction for assessment of biomarkers in early respiratory drug development

Robert Lins et al. Int J Clin Pharmacol Ther. 2019 Jan.

Abstract

Objective: To study the success rate and reproducibility of sputum induction (SI) in healthy subjects (HS) and asthma patients (AP).

Materials and methods: 130 HSs/APs were recruited for early-phase studies to evaluate sputum biomarkers. SI and sample processing were performed according to standard protocols.

Results: Adequate samples were obtained from 46% of HSs and 80% of APs. There was no difference between the groups with adequate and nonadequate samples. Differences between HS and AP groups were minor. Reproducibility was 56.0% in HSs and 90.6% in APs.

Conclusion: Sputum induction for biomarker assessment in early drug development is feasible in HSs and APs, but only modestly reproducible in HSs. The technique is complex and time consuming. .

PubMed Disclaimer