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Comment
. 2018 Nov 20:9:1565.
doi: 10.3389/fphys.2018.01565. eCollection 2018.

Commentary: Voltage Gating of Mechanosensitive PIEZO Channels

Lars Kaestner  1   2 , Stephane Egee  3   4
Affiliations
Comment

Commentary: Voltage Gating of Mechanosensitive PIEZO Channels

Lars Kaestner et al. Front Physiol. .
No abstract available

Keywords: Hereditary Xerocytosis; Piezo1; erythrocyte; non-selective voltage-dependent cation channel; patch-clamp.

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Figures

Figure 1
Figure 1
Comparison of the non-selective voltage activated cation channel recorded in red blood cells (A,C) and Piezo1 recorded in overexpressing Neuro2A cells (B,D). (A) Current traces of the non-selective voltage-dependent cation channel in inside-out patches of red blood cells in Na-tartrate-solution in mM (bath solution: 70 Na-tartrate, 2.5 BaCl2, 10 MOPS, 10 glucose, 75 saccharose, pH = 7.4; pipette solution: 20 Na-tartrate, 2.5 BaCl2, 10 MOPS, 10 glucose, 225 saccharose, pH = 7.4). (B) Current traces of Piezo1 in outside-out patches of overexpressing Neuro2A cells in symmetrical NaCl-solution in mM (140 NaCl, 10 HEPES, 5 EGTA, pH = 7.4). The conductance of the channels presented in (A,B) is 21 ± 5 and 27.1 ± 1.2 pS, respectively. (C) The open state probability as function of the membrane potential. In both series (open symbols and filled symbols), the open probability was calculated from 3 min of continuous recording at each potential. The curves were drawn by eye. (D) Tail currents from individual cells were normalized to their maximum and fitted to a Boltzmann relationship. Pooled data are shown as mean ± SEM. (A,C) are reproduced from Kaestner et al. (1999, 2000), respectively and (B,D) from Moroni et al. (2018).
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References

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