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. 2019 Mar 15:125:221-231.
doi: 10.1016/j.ijbiomac.2018.12.033. Epub 2018 Dec 6.

A rapid, accurate and sensitive method for determination of monosaccharides in different varieties of Osmanthus fragrans Lour by pre-column derivatization with HPLC-MS/MS

Baolei Fan  1 , Tingting Li  1 , Xiaofang Song  1 , Chao Wu  1 , Chunqi Qian  2
Affiliations

A rapid, accurate and sensitive method for determination of monosaccharides in different varieties of Osmanthus fragrans Lour by pre-column derivatization with HPLC-MS/MS

Baolei Fan et al. Int J Biol Macromol. .

Abstract

A specific HPLC-MS/MS (High-Performance Liquid Chromatography with tandem Mass Spectrometry) method was developed and validated for simultaneous determination of several monosaccharides in three kinds Osmanthus fragrans Lour. After extraction, separation, protein removal, pigment removal and hydrolysis, monosaccharides was finally obtained from Osmanthus fragrans Lour. Positive ion mode detection and Multiple Reaction Monitoring (MRM) mode were used for quantitative analysis by PMP pre-column derivatization and Electrospray Ionization (ESI). Analysis and content determination of 6 monosaccharide components in 3 kinds of Osmanthus fragrans Lour. The HPLC separation was achieved on a Shim-pack VP-ODS6022748 (150 L × 2.0) with gradient elution at a flow rate of 0.2 ml/min in a run time of 40 min, and the mobile phase was acetonitrile-5 mmol/L ammonium acetate. PMP derivatization in HPLC-MS/MS can accurately measure Osmanthus fragrans Lour. mannose (Man), ribose (Rib), rhamnose (Rha), galacturonic acid (Gal UA), glucose (Glu), galactose (Gal), xylose (Xyl), fucose (Fuc). The results showed that HPLC-MS/MS pre-column derivatization method was simple and rapid, with small measurement error, but high sensitivity and good repeatability. The analysis of monosaccharide components in polysaccharide components has important practical significance.

Keywords: HPLC-MS/MS; Osmanthus fragrans Lour.; PMP pre-column derivatization.

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Conflict of interest statement

Conflicts of interest

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Q3 Scan of PMP-labeled monosaccharide standard. a-Man, b-Rib, c-Glu, d-Gal, e-Xyl, f-Fuc
Fig. 1.
Fig. 1.
Q3 Scan of PMP-labeled monosaccharide standard. a-Man, b-Rib, c-Glu, d-Gal, e-Xyl, f-Fuc
Fig. 2.
Fig. 2.
Product Ion Scan of PMP-labeled monosaccharide standard. a-Man, b-Rib, c-Glu, d-Gal, e-Xyl, f-Fuc.
Fig. 3.
Fig. 3.
PMP derivatization of glucose standards and mass spectrometry fragmentation of PMP-labeled glucose standard derivatives in positive ion mode.
Fig. 4.
Fig. 4.
The SD of the content of each monosaccharide in Osmanthus fragrans var. semperflorens is indicated by error bars (n = 6).
Fig. 5.
Fig. 5.
The SD of the content of each monosaccharide in Osmanthus fragrans scv. Latifoliu is indicated by error bars (n = 6).
Fig. 6.
Fig. 6.
The SD of the content of each monosaccharide in Osmanthus fragrans var. thunbergii. is indicated by error bars (n = 6).
Fig. 7.
Fig. 7.
Multiple reaction detection chromatogram ofPMP-labeled mixed monosaccharide standard in positive ion mode. 1-mannose, 2-ribose, 3-glucose, 4-galactose, 5-xylose, 6-fucose.
Fig. 8.
Fig. 8.
MRM ofPMP-labeled Osmanthus fragrans var. semperflorens in positive ion mode. 1-mannose, 2-ribose, 3-rhamnose, 4-galacturonic acid, 5-glucose, 6-galactose, 7-xylose, 8-fucose.
Fig. 9.
Fig. 9.
MRM ofPMP-labeled Osmanthus fragrans scv. latifoliu in positive ion mode. mannose, 2-ribose, 3-rhamnose, 4-galacturonic acid, 5-glucose, 6-galactose, 7-xylose, 8-fucose.
Fig. 10.
Fig. 10.
MRM of PMP-labeled Osmanthus fragrans var. thunbergii. in positive ion mode. 1-mannose, 2-ribose, 3-rhamnose, 4-galacturonic acid, 5-glucose, 6-galactose, 7-xylose, 8-fucose.
Fig. 11.
Fig. 11.
Effect of PMP concentration on derivative results.
See this image and copyright information in PMC

References

    1. Ouyang XL, Wei LX, Wang HS, Pan YM, Antioxidant activity and phytochemical composition of Osmanthus’ pulps, S. Afr. J. Bot 98 (2015) 162–166.
    1. Huang B, Chen H, Shao L, The ethanol extract of Osmanthus fragrans attenuates Porphyromonas gingivalis lipopolysaccharide-stimulated inflammatory effect through the nuclear factor erythroid 2-related factormediated antioxidant signalling pathway, Arch. Oral Biol 60 (7) (2015) 1030–1038. - PubMed
    1. Xiong L, Mao S, Lu B, Yang J, Zhou F, Hu Y, et al., Osmanthus fragrans flower extract and acteoside protect against D-galactose-induced aging in an ICR mouse model, J. Med. Food 19 (1) (2016) 54–61. - PubMed
    1. Wu L, Chang L, Chen S, Fan N, Ho J. Annie, Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: a potential natural and functional food flavor additive, LWT Food Sci. Technol 42 (9) (2009) 1513–1519.
    1. Xiong L, Yang J, Jiang Y, Lu B, Hu Y, Zhou F, et al., Phenolic compounds and antioxidant capacities of 10 common edible flowers from China, J. Food Sci 79 (4) (2014) 517–525. - PubMed

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