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. 2018 Nov 7:2018:1931634.
doi: 10.1155/2018/1931634. eCollection 2018.

Subcritical Water Extraction of Chlorella pyrenoidosa: Optimization through Response Surface Methodology

Selvakumar Thiruvenkadam  1 , Shamsul Izhar  1 , Yoshida Hiroyuki  1 , Razif Harun  1
Affiliations

Subcritical Water Extraction of Chlorella pyrenoidosa: Optimization through Response Surface Methodology

Selvakumar Thiruvenkadam et al. Biomed Res Int. .

Abstract

Subcritical water extraction (SCW) was used to extract oil from Chlorella pyrenoidosa. The operational factors such as reaction temperature, reaction time, and biomass loading influence the oil yield during the extraction process. In this study, response surface methodology was employed to identify the desired extraction conditions for maximum oil yield. Experiments were carried out in batch reactors as per central composite design with three independent factors including reaction temperature (170, 220, 270, 320, and 370°C), reaction time (1, 5, 10, 15, and 20 min), and biomass loading (1, 3, 5, 10, and 15%). A maximum oil yield of 12.89 wt.% was obtained at 320°C and 15 min, with 3% biomass loading. Sequential model tests showed the good fit of experimental data to the second-order quadratic model. This study opens the great potential of SCW to extract algal oil for use in algal biofuel production.

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Figures

Figure 1
Figure 1
Schematic diagram of the experimental apparatus: (a) salt bath and (b) batch reactor.
Figure 2
Figure 2
Response surface plots of oil yield (g/g algae) at given (a) reaction temperature (°C), (b) reaction time (min), and (c) biomass loading (%).
Figure 3
Figure 3
The effect of (a) reaction temperature, (b) reaction time, and (c) biomass loading on the oil yield.
See this image and copyright information in PMC

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