Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Nov 11:2018:1614683.
doi: 10.1155/2018/1614683. eCollection 2018.

Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility

Chenyue Qian  1 , Heming Guo  2 , Xiaohong Chen  2 , Aiming Shi  1 , Sicheng Li  2 , Xin Wang  3 , Jie Pan  1 , Chen Fang  2
Affiliations

Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility

Chenyue Qian et al. J Diabetes Res. .

Abstract

Aims: The programmed death- (PD-) 1/PD-1 ligand (PD-L) pathway plays an important role in regulating T cell activation and maintaining peripheral tolerance. Accumulated studies showed that PD-1/PD-L1 pathway was involved in the development of type 1 diabetes (T1DM). Since the genetic background of type 1 diabetes differs greatly among the different population, we aim to investigate the association of genetic polymorphisms in PD-1 and PD-L1 with T1DM susceptibility in Chinese population.

Methods: In total, 166 T1DM patients and 100 healthy controls were enrolled into the study. Genomic DNA was extracted from 4 mL peripheral blood samples collected from each subject. Genotyping of 8 selected SNPs of PD-1 and PD-L1 was carried out by the pyrosequencing PSQ 24 System using PyroMark Gold reagents (QIAGEN).

Results: SNP rs4143815 in PD-L1 was significantly associated with T1DM. People carrying the C allele of rs4143815 suffering less risk of T1DM and T1DM patients with G/G genotype showed higher levels of autoantibody (AAB) positive incidence compared with C allele carriers. No significant associations were found in other SNPs.

Conclusions: Our results indicate that rs4143815 of PD-L1 is significantly associated with T1DM and may serve as a new biomarker to predict the T1DM susceptibility.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The secondary structure of partial PD-L1 3′UTR mRNA with different allele carrier of rs4143815 using RNAfold (http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi).
See this image and copyright information in PMC

References

    1. Tian J., Liu Y., Liu Y., Chen K., Lyu S. Cellular and molecular mechanisms of diabetic atherosclerosis: herbal medicines as a potential therapeutic approach. Oxidative Medicine and Cellular Longevity. 2017;2017:16. doi: 10.1155/2017/9080869.9080869 - DOI - PMC - PubMed
    1. Fife B. T., Bluestone J. A. Control of peripheral T-cell tolerance and autoimmunity via the CTLA-4 and PD-1 pathways. Immunological Reviews. 2008;224(1):166–182. doi: 10.1111/j.1600-065X.2008.00662.x. - DOI - PubMed
    1. Fujisawa R., Haseda F., Tsutsumi C., et al. Low programmed cell death-1 (PD-1) expression in peripheral CD4+ T cells in Japanese patients with autoimmune type 1 diabetes. Clinical & Experimental Immunology. 2015;180(3):452–457. doi: 10.1111/cei.12603. - DOI - PMC - PubMed
    1. Nolan E., Savas P., Policheni A. N., et al. Combined immune checkpoint blockade as a therapeutic strategy for BRCA1-mutated breast cancer. Science Translational Medicine. 2017;9(393, article eaal4922) doi: 10.1126/scitranslmed.aal4922. - DOI - PMC - PubMed
    1. Bang A., Wilhite T. J., Pike L. R. G., et al. Multicenter evaluation of the tolerability of combined treatment with PD-1 and CTLA-4 immune checkpoint inhibitors and palliative radiation therapy. International Journal of Radiation Oncology, Biology, Physics. 2017;98(2):344–351. doi: 10.1016/j.ijrobp.2017.02.003. - DOI - PubMed

LinkOut - more resources