doi: 10.1007/978-1-4939-8861-7_12.
Methods to Probe Calcium Regulation by BCL-2 Family Members
Affiliations
- PMID: 30536006
- DOI: 10.1007/978-1-4939-8861-7_12
Item in Clipboard
Methods to Probe Calcium Regulation by BCL-2 Family Members
Methods Mol Biol.
2019.
Abstract
BCL-2 family members have additional roles beyond direct regulation of mitochondrial outer membrane permeabilization (MOMP) in apoptosis. One such important function is the release of calcium from the endoplasmic reticulum (ER), which critically contributes to the process of apoptosis. Here, we describe a protocol to measure calcium levels in the ER, mitochondria, and cytosol, with specific consideration of BCL-2 family biology.
Keywords: Apoptosis; BCL-2 Family; Calcium; Endoplasmic reticulum; Mitochondria.
Similar articles
-
Mitochondria in neutrophil apoptosis.Int J Hematol. 2006 Oct;84(3):199-204. doi: 10.1532/IJH97.06131. Int J Hematol. 2006. PMID: 17050191 Review.
-
Endoplasmic reticulum stress induces calcium-dependent permeability transition, mitochondrial outer membrane permeabilization and apoptosis.Oncogene. 2008 Jan 10;27(3):285-99. doi: 10.1038/sj.onc.1210638. Epub 2007 Aug 13. Oncogene. 2008. PMID: 17700538
-
Balancing life and death: BCL-2 family members at diverse ER-mitochondrial contact sites.FEBS J. 2022 Nov;289(22):7075-7112. doi: 10.1111/febs.16241. Epub 2021 Nov 2. FEBS J. 2022. PMID: 34668625 Review.
-
Effects of targeted Bcl-2 expression in mitochondria or endoplasmic reticulum on renal tubular cell apoptosis.Am J Physiol Renal Physiol. 2008 Mar;294(3):F499-507. doi: 10.1152/ajprenal.00415.2007. Epub 2007 Dec 26. Am J Physiol Renal Physiol. 2008. PMID: 18160625
-
BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.Cell. 2016 Apr 7;165(2):421-33. doi: 10.1016/j.cell.2016.02.026. Epub 2016 Mar 3. Cell. 2016. PMID: 26949185 Free PMC article.
Cited by
-
BOK controls apoptosis by Ca2+ transfer through ER-mitochondrial contact sites.Cell Rep. 2021 Mar 9;34(10):108827. doi: 10.1016/j.celrep.2021.108827. Cell Rep. 2021. PMID: 33691099 Free PMC article.
-
The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores.ASN Neuro. 2022 Jan-Dec;14:17590914221076698. doi: 10.1177/17590914221076698. ASN Neuro. 2022. PMID: 35103506 Free PMC article.
-
Compound Porcine Cerebroside and Ganglioside Injection (CPCGI) Attenuates Sevoflurane-Induced Nerve Cell Injury by Regulating the Phosphorylation of p38 MAP Kinase (p38MAPK)/Nuclear Factor kappa B (NF-κB) Pathway.Med Sci Monit. 2020 Mar 1;26:e919600. doi: 10.12659/MSM.919600. Med Sci Monit. 2020. PMID: 32114591 Free PMC article.
-
Regulation of Cell Death by Mitochondrial Transport Systems of Calcium and Bcl-2 Proteins.Membranes (Basel). 2020 Oct 21;10(10):299. doi: 10.3390/membranes10100299. Membranes (Basel). 2020. PMID: 33096926 Free PMC article. Review.
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
