Identification of enterotoxigenic Escherichia coli isolates with enzyme-labeled synthetic oligonucleotide probes
- PMID: 3053766
- PMCID: PMC266839
- DOI: 10.1128/jcm.26.10.2173-2176.1988
Identification of enterotoxigenic Escherichia coli isolates with enzyme-labeled synthetic oligonucleotide probes
Abstract
Commercially available kits containing alkaline phosphatase-labeled oligonucleotide probes for Escherichia coli heat-stable enterotoxins (STI-H, STI-P, and STII) and the heat-labile enterotoxin were compared with bioassays and radiolabeled recombinant DNA probes to identify enterotoxigenic E. coli from 100 clinical isolates. There was very good agreement between the three methods.
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