Distribution of some Gal beta 1-3(4)GlcNAc related carbohydrate antigens on the mouse uterine epithelium in relation to the peri-implantational period

Abstract

Using monoclonal antibodies of defined carbohydrate specificity we have looked at the distribution of various Gal beta 1-3(4)GlcNAc related oligosaccharide determinants in the mouse uterus during the first 6 days of pregnancy. Frozen sections of uterus from B6D2F1, B6CBF1 or B6D2F1/BOM female mice were incubated with the monoclonal antibodies and then with a fluorescein isothiocyanate conjugate of goat anti-mouse IgM and viewed by epifluorescence illumination. None of the antibodies bound specifically to stroma cells but antibodies recognising difucosylated Gal beta 1-3(4)GlcNAc structures, the monofucosylated type II determinant (SSEA-1) and an H type I oligosaccharide bound to cells of the uterine luminal epithelium and glands and to the uterine secretions. Antibodies recognising the three different types of saccharide showed independent changes in staining intensity during early pregnancy. The antibody which recognises H type I structures (667/9E9) showed a change in distribution from binding to most cells of the uterine epithelium in the non-pregnant mouse and on day 3 of pregnancy to binding restricted to areas of epithelial cells interspersed with non-staining clumps of cells between days 4 and 5 of pregnancy.