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. 2018 Dec 12;18(1):642.
doi: 10.1186/s12879-018-3538-2.

Arboviruses isolated from the Barkedji mosquito-based surveillance system, 2012-2013

El Hadji Ndiaye  1 , Diawo Diallo  2 , Gamou Fall  3 , Yamar Ba  1 , Ousmane Faye  3 , Ibrahima Dia  1 , Mawlouth Diallo  1
Affiliations

Arboviruses isolated from the Barkedji mosquito-based surveillance system, 2012-2013

El Hadji Ndiaye et al. BMC Infect Dis. .

Abstract

Background: A mosquito-based arbovirus surveillance system was set up at Barkedji, Senegal after the first outbreak of Rift valley fever in West Africa in 1988. This system was recently updated using more sampling methods and collecting in greater number of ponds and villages sites.

Methods: For the current study, mosquitoes were sampled biweekly between July and December 2012 and 2013 using CDC+CO2 light traps set at ground and canopy level, mosquito nets baited with goat, sheep, human or chicken, light traps baited with goat, sheep and chicken; bird-baited traps using pigeons or chickens placed either at the ground or canopy level. Collected mosquitoes were identified, pooled and screened for arboviruses.

Results: A total of 42,969 mosquitoes in 4,429 pools were processed for virus isolation. Ten virus species were identified among 103 virus isolates. West Nile virus (WNV; 31 isolates), Barkedji virus (BARV; 18), Sindbis virus (SINV; 13), Usutu virus (USUV; 12), Acado virus (ACAV; 8), Ndumu virus (NDUV; 9), Sanar virus (SANV; 7), Bagaza virus (BAGV; 3), Rift valley fever virus (RVFV; 1), and Yaounde virus (YAOV; 1) were isolated from 9 ponds (91 strains) and 7 villages (12 strains). Only 3 virus species (WNV, NDU and SINV) were isolated from villages. The largest numbers of isolates were collected in October (29.1% of total isolates) and November (50.5%). Viruses were isolated from 14 mosquito species including Cx. neavei (69.9% of the strains), Cx. antennatus (9.7%), and Ma. uniformis (4.8%). NDUV, ACAV, and SINV are herein reported for the first time in the Barkedji area. Isolation of ACAV and SANV from a pool of male Ma. uniformis and USUV and BARV from a pool of male Cx. neavei, are reported for the first time to our knowledge.

Conclusion: Our data indicate that the Barkedji area is characterized by a high diversity of viruses of medical, veterinary and unknown importance. Arboviruses were first detected in July at the beginning of the rainy season and peaked in abundance in October and November. The Barkedji area, an enzootic focus of several potentially emerging arboviruses, should be surveilled annually to be prepared to deal with future disease emergence events.

Keywords: Aampling methods; Arbovirus; Mosquitoes; Ponds; Surveillance; Villages.

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Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Figures

Fig. 1
Fig. 1
Study area with the names of villages and ponds sampled and their geographic coordinates in UTM (Modified from Google Earth)
Fig. 2
Fig. 2
Temporal dynamics of mosquito vectors of arboviruses in the Barkedji area, 2012-2013
Fig. 3
Fig. 3
Spatial distribution of arboviruses detected in ponds and villages of the Barkedji area, 2012-2013. Saaka pond, from which Barkedji and West Nile viruses were isolated (1 pool each) is not included in the map because it is located about 50Km from the other sites
See this image and copyright information in PMC

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