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. 2018 Dec;16(6):4527-4536.
doi: 10.3892/etm.2018.6818. Epub 2018 Oct 1.

Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D-LC-MS/MS

Yanyun Cao  1 , Shunming Xu  1 , Wei Kong  1 , Haibin Cai  1 , Yang Xu  1
Affiliations

Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D-LC-MS/MS

Yanyun Cao et al. Exp Ther Med. 2018 Dec.

Abstract

Chronic spontaneous urticaria (CSU) is one of the most common types of chronic urticaria (CU), with symptoms that recur easily, migrate and are refractory. It is unclear whether association between the differentiation of protein expression levels in the serum of CSU patients and the different duration of wheals exists. In the present study the samples were divided according to the duration of the wheals into group A (wheal duration <2 h) and group B (wheal duration 12-24 h). Differentially expressed proteins in sera of CSU patients with different durations of wheals were identified and validated with isobaric tags for relative and absolute quantitation (iTRAQ) in combination with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS). Three hundred and seventy CSU serum-related proteins were initially identified. Among these proteins, ~30 had significant differences between the groups. According to the classification of biological functions and upregulated/downregulated values, serum amyloid A (SAA), CFL1, TPM4 and monocyte differentiation antigen (CD14) were chosen and validated by enzyme-linked immunosorbent assay (ELISA). The expression levels of CD14 in sera were not significantly different among the groups. SAA, CFL1 and TPM4 were associated with the wheal duration in CSU patients and therefore could be considered as new potential inflammatory biomarkers associated with CSU.

Keywords: chronic spontaneous urticaria; cofilin-1; isobaric tags for relative and absolute quantitation; serum amyloid A protein.

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Figures

Figure 1.
Figure 1.
(A and B) The subcellular location and functional distribution of differentially expressed proteins of group A and B. Group A: wheal duration <2 h, 10 cases; group B: wheal duration 12–24 h, 10 cases. Red, upregulated; black, downregulated. CRP, C-reactive protein.
Figure 2.
Figure 2.
(A and B) The subcellular location and functional distribution of differentially expressed proteins between groups B and D. Group B: wheal duration 12–24 h, 10 cases; group D: healthy control group, 10 cases. Red, upregulated; black, downregulated. CRP, C-reactive protein.
Figure 3.
Figure 3.
(A and B) The subcellular location and function distribution of differentially expressed proteins between group A and D. Group A: wheal duration <2 h, 10 cases; group D: healthy control group, 10 cases. Red, upregulated; black, downregulated. CRP, C-reactive protein.
Figure 4.
Figure 4.
(A) The SAA, (B) the CFL1, (C) the TPM4, and (D) the CD14 levels in serum of CSU patients of different groups by ELISA. Group Y: wheal duration <2 h, 21 cases; group G: wheal duration 12–24 h, 21 cases; group J: healthy control group, 21 cases. Statistical significance, P<0.05. SAA, serum amyloid A; CSU, chronic spontaneous urticaria; ELISA, enzyme-linked immunosorbent assay.
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