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. 2018 Dec;16(6):4791-4795.
doi: 10.3892/etm.2018.6750. Epub 2018 Sep 17.

Long non-coding RNA HOTAIR overexpression improves premature ovarian failure by upregulating Notch-1 expression

Wei Zhao  1 , Liwei Dong  1
Affiliations

Long non-coding RNA HOTAIR overexpression improves premature ovarian failure by upregulating Notch-1 expression

Wei Zhao et al. Exp Ther Med. 2018 Dec.

Abstract

The aim of the present study was to investigate the role of long non-coding (lnc)RNA HOTAIR in premature ovarian failure. A total of 69 women with spontaneous premature ovarian failure and 48 healthy women were enrolled in the present study. Ovarian tissues and blood samples were harvested from each participant. The expression of HOTAIR in ovarian tissues and sera was detected using reverse transcription-quantitative polymerase chain reaction. A receiver operator characteristic curve analysis was performed to assess the diagnostic value of HOTAIR expression in ovarian tissues and sera for premature ovarian failure. Hamster ovary cell lines overexpressing HOTAIR were established and cell apoptosis were assessed using an MTT assay. The effect of HOTAIR upregulation on the expression of Notch-1 was measured using western blotting. The expression of HOTAIR in ovarian tissues and serum samples was significantly lower in patients with premature ovarian failure compared with healthy controls. These results suggest that ovarian and serum HOTAIR expression levels may be used to accurately predict the risk of premature ovarian failure. It was also demonstrated that HOTAIR overexpression upregulates Notch-1 protein expression in hamster ovary cells and reduced apoptosis, whereas the Notch inhibitor L685458 ameliorated these effects. In conclusion, the results of the present study suggest that LncRNA HOTAIR overexpression improves premature ovarian failure by upregulating the expression of Notch-1.

Keywords: Notch-1; apoptosis; diagnosis; long non-coding RNA HOTAIR; premature ovarian failure.

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Figures

Figure 1.
Figure 1.
HOTAIR expression in ovarian tissues from patients with premature ovarian failure and healthy controls. *P<0.05 vs. control.
Figure 2.
Figure 2.
Serum HOTAIR expression in patients with premature ovarian failure and healthy controls. *P<0.05 vs. control.
Figure 3.
Figure 3.
Diagnostic value of HOTAIR expression. Diagnostic value of HOTAIR expression in (A) ovarian tissues and (B) serum for premature ovarian failure. HOTAIR expression data were obtained through reverse transcription-quantitative polymerase chain reaction. The true positive rate (sensitivity) was plotted against the false positive rate (1-specificity). The cut-off value for HOTAIR in ovarian tissues was 0.1221. The cut-off value for HOTAIR in serum was 0.1140. Solid circles represent diagnostic curve. Squares represent the line of identity.
Figure 4.
Figure 4.
HOTAIR overexpression promoted Notch-1 protein expression in (A) Lec8 and (B) CHO normal hamster ovary cell lines. *P<0.05. C, non-transfected cells; NC, negative control (cells transfected with empty vectors); Over, overexpression.
Figure 5.
Figure 5.
Apoptosis of hamster ovary cells under different conditions. Notch-1 expression in (A) Lec8 and (B) CHO cells overexpressing HOTAIR and treated with or without Notch inhibitor L685458. *P<0.05.
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