LncRNA HOTAIR improves diabetic cardiomyopathy by increasing viability of cardiomyocytes through activation of the PI3K/Akt pathway

Abstract

The current study aimed to investigate the role of long non-coding RNA (lncRNA) homeobox transcript antisense RNA (HOTAIR) in the pathogenesis of diabetic cardiomyopathy. Patients with diabetic cardiomyopathy, patients with diabetes but without cardiomyopathy and healthy controls were included in the current study. All participants underwent myocardial biopsy to collect myocardial tissues. Blood samples were also collected from each participant to prepare serum. Expression of HOTAIR in myocardial tissues was detected by reverse transcription-quantitative polymerase chain reaction. Receiver operating characteristic curve analysis was performed to evaluate the diagnostic value of serum HOTAIR for diabetic cardiomyopathy. AC16 human cardiomyocyte cells were treated with high glucose to observe the changes in expression of HOTAIR and phosphorylation of Akt. HOTAIR expression vector was transfected into cells of AC16 cell line and the effects of HOTAIR overexpression on cell viability and Akt phosphorylation were detected by MTT assay and western blot analysis, respectively. HOTAIR expression was significantly downregulated in myocardial tissues and serum of patients with diabetic cardiomyopathy compared with patients with diabetes and healthy controls. Serum HOTAIR could be used to effectively distinguish patients with diabetic cardiomyopathy from healthy controls. High glucose treatment inhibited HOTAIR expression and Akt phosphorylation. HOTAIR overexpression promoted Akt phosphorylation. HOTAIR overexpression improved AC16 cell viability, while PI3K/Akt inhibitor treatment reduced this effect. LncRNA HOTAIR may improve diabetic cardiomyopathy by increasing the viability of cardiomyocytes through activation of the PI3K/Akt pathway.

Keywords: activation; diabetic cardiomyopathy; homeobox transcript antisense RNA; long non-coding RNA; phosphoinositide 3-kinase/Akt; viability.

Figure 1.

Expression of HOTAIR in different groups of participants. The figure indicates HOTAIR expression in (A) myocardial tissues and (B) serum of different groups of participants. DC, diabetic cardiomyopathy; D, diabetes without cardiomyopathy; HOTAIR, homeobox transcript antisense RNA. *P<0.05.

Figure 2.

Diagnostic values of HOTAIR expression for diabetic cardiomyopathy. This figure indicates the receiver operating characteristic curve for the diagnosis of diabetic cardiomyopathy using HOTAIR expression in (A) myocardial tissues and (B) serum. HOTAIR, homeobox transcript antisense RNA.

Figure 3.

Effects of high glucose on HOTAIR expression and Akt phosphorylation in human cardiomyocytes. The figure indicates the effects of high glucose on (A) HOTAIR expression and (B) Akt phosphorylation in human cardiomyocytes. *P<0.05. HOTAIR, homeobox transcript antisense RNA; p-, phosphorylated.

Figure 4.

Effect of HOTAIR overexpression on Akt phosphorylation in human cardiomyocytes. *P<0.05. C, control (non-transfected); NC, negative control (empty vector); Over, HOTAIR overexpression (transfected with overexpression vector); HOTAIR, homeobox transcript antisense RNA; p-, phosphorylated.

Figure 5.

Effect of HOTAIR expression on cell viability. *P<0.05. C, control (non-transfected); NC, negative control (empty vector); Over, HOTAIR overexpression (transfected with overexpression vector); HOTAIR, homeobox transcript antisense RNA.