Type II Na+-phosphate Cotransporters and Phosphate Balance in Teleost Fish

Abstract

Teleost fish are excellent models to study the phylogeny of the slc34 gene family, Slc34-mediated phosphate (P_i) transport and how Slc34 transporters contribute P_i homeostasis. Fish need to accumulate P_i from the diet to sustain growth. Much alike in mammals, intestinal uptake in fish is partly a paracellular and partly a Slc34-mediated transcellular process. Acute regulation of P_i balance is achieved in the kidney via a combination of Slc34-mediated secretion and/or reabsorption. A great plasticity is observed in how various species perform and combine the different processes of secretion and reabsorption. A reason for this diversity is found in one or two whole genome duplication events followed by potential gene loss; consequently, teleosts exhibit distinctly different repertoires of Slc34 transporters. Moreover, due to habitats with vastly different salinity, teleosts face the challenge of either preserving water in a hyperosmotic environment (seawater) or excreting water in hypoosmotic freshwater. An additional challenge in understanding teleost P_i homeostasis are the genome duplication and retention events that diversified peptide hormones such as parathyroid hormone and stanniocalcin. Dietary P_i and non-coding RNAs also regulate the expression of piscine Slc34 transporters. The adaptive responses of teleost Slc34 transporters to e.g. P_i diets and vitamin D are informative in the context of comparative physiology, but also relevant in applied physiology and aquaculture. In fact, P_i is essential for teleost fish growth but it also exerts significant adverse consequences if over-supplied. Thus, investigating Slc34 transporters helps tuning the physiology of commercially valuable teleost fish in a confined environment.

Keywords: Aquaculture; Dietary phosphate (Pi) regulation; Epithelial phosphate transport; Hormonal regulation; Post-transcriptional antisense regulation; Slc34; Teleost fish; Whole genome duplication.